Figure 1

Induction of micronuclei in HCMV UL76-expressing human glioblastoma (U-373 MG) cells. (A) Western blot analysis of UL76 protein in cells. α-tubulin was used as a loading control. (B) Representative images of micronuclei (arrow) detected by DAPI staining of U-373 MG cells stably transfected with control vector (-UL76), or with a plasmid expressing UL76 (+UL76). (C) Quantification of micronuclei in an asynchronous cell population expressing either vector (P7) or UL76 (S1, S3, S4, and S5). The average data point was calculated from three independent experiments. At least 4000 cells were counted for each cell line.