Figure 2

Induction of chromosomal misalignments in the HCMV UL76-expressing glioblastoma cells (U-373 MG). (A) Representative images of chromosomal misalignments induced in cells stably expressing HCMV UL76; chromosomal laggings (ii and iii), and mitotic bridges (iii to v) in glioblastoma cells constitutively expressing HCMV UL76. In parallel control experiments, empty vector-transfected cells were used as normal mitotic cells (i). Chromosomes were stained with DAPI (blue) and mitotic cells were visualized by immunofluorescent staining using a monoclonal antibody to α-tubulin (red). Two side-by-side panels of single-labeled immunofluorescent images and a third panel with an overlapping image are shown. (B) Quantification of chromosomal misalignments in mitotic cells stably expressing HCMV UL76 (S1, S3, S4, and S5) or control cells (P7). The average data point was calculated from three independent experiments. At least 800 mitotic cells were counted for each cell line.