Figure 4

Representative images of supernumerary centrosomes at the mitotic stage in UL76-expressing cells. (A) Centrosomes were visualized by staining with γ-tubulin (red) and the chromosomes were visualized by DAPI stain (blue). Normal bipolar centrosome (i) at the mitotic stage is shown. Abnormal centrosome images, tricentrosome (ii) and tetracentrosome (iii) in mitotic cells. Two side-by-side panels of single labeled immunofluorescent images and a third panel with an overlapping image are shown. (B) Quantification of mitotic cells with supernumerary centrosomes in cells stably expressing HCMV UL76 (S1, S3, S4, and S5) and control vector (P7).