Figure 5

UL76 induces γ-H2AX foci. (A) Western blot analysis of γ-H2AX protein levels in cells. α-tubulin was used as a loading control. (B) Quantification of the increase of γ-H2AX protein levels in U-373 MG cells stably expressing UL76 (S1, S3, S4, and S5) after normalization against the α-tubulin loading control and P7 control cells. FI: fold increase. (C) Representative image of γ-H2AX foci (green) in control P7 cells (-UL76) or in UL76-expressing cells (+UL76). Nuclei were counterstained with DAPI (blue). Magnification of the foci images is shown. (D) Quantification of high focal γ-H2AX staining in cells stably-expressing UL76 (S1, S3, S4, and S5) compared with control P7 cells. The average data point was calculated from three independent experiments. At least 1000 cells were counted for each cell line.