Figure 1

The Noggin transgene is expressed in P-cadherin positive hair progenitor cells in Nse-Noggin mice from late embryonic stages onward. (A) Schematic structure of Nse-Noggin transgene. (B-D) typical images of tail skin of Nse-Noggin mice show the specific transgene (GFP) expression pattern. GFP positive cells are detected in hair buds from E16 (B) In postnatal animals (P0(C) & P6(D)) transgene expression is also observed only in subpopulations of hair follicles (C&D). (E-F) double staining confirms that the transgene is expressed in hair progenitors (P-cadherin Positive, E-E") but not in fully differentiated epithelium (K14 positive, F). (E) shows typical GFP/P-cadherin double staining of a hair follicle from an adult Nse-Noggin animal. E'& E" are the split channels of P-cadherin and GFP, respectively. (F) shows typical GFP/K14 double staining of a hair follicle from an E18 Nse-Noggin mouse. (G) shows typical NSE staining of a hair follicle from an adult WT animal. Note that NSE is highly expressed in a subpopulation of hair follicle cells. (H-H") shows typical GFP/NSE double staining of well developed tumor section. H'& H" are the split channels of NSE and GFP, respectively. Bar = 40 μm.