Figure 6

Western blot analysis confirms that expression of CD44 and P-cad is differentially regulated. (A)Western Blotting using skin lysates from Nse-BMP4, WT and Nse-Noggin mice. Each lane represents lysate from an individual animal. Nse-BMP4 (lane 1-3), WT (lane 4-6), Nse-Noggin (lane 7-9). Lane 7 represents lysate from a well-developed tumor, while lane 8&9 represent pre-neoplasia lysates from two individual Nse-Noggin mice. Top panel shows CD44 expression. Notice that CD44 is not up-regulated in Nse-Noggin tumors generally, which is consistent with the double-staining finding in (Figure 3D). Middle panel shows P-cad expression. Note that P-cad expression is dramatically up-regulated only in well-developed tumor. (B&C) quantify the protein expression of P-cad and CD44, respectively. *, differs from WT by ANOVA at p < 0.01.