Figure 2

Concentrations of IL-12 and IFN-γ in right (R) and left (L) hemispheres of rats treated with PBS or rAAV2/IL-12 and with tumor implantation. Concentrations of IL-12 (A) and IFN-γ (C) were measured on the last day of week 1, 2, and 3 post tumor implantation (i.e., on the last day of week 3, 4, and 5 post viral injection). The concentrations of IL-12 (A) and IFN-γ (C) with tumor transplantation were compared to those of IL-12 (B) and IFN-γ (D) with no tumor implantation (no). Note that both concentrations with no tumor implantation were taken from the data in Figure 1. Photographs of 2 mm thickness brain sections (E) show rAAV2/IL12-treated (left 3 columns) and PBS-treated (right 3 columns) brains harvested on the last day of week 1 (W1), week 2 (W2), and week 3 (W3) post tumor implantation. Black arrows indicate the maximal tumor size and this site was taken for analyzing the concentration of IL-12 and IFN-γ. PBS LH (diamond): left hemisphere from the PBS-treated; PBS RH (triangle): right hemisphere from the PBS-treated; AAV2/IL12 RH (round): right hemisphere from the rAAV2/IL12-treated; AAV2/IL12 LH (square): left hemisphere from the rAAV2/IL12-treated; AAV2/IL12 R(no) (empty square): right hemisphere of rAAV2/IL12-treated brain without tumor implantation; AAV2/IL12 L(no) (empty round): left hemisphere from the rAAV2/IL12-treated brain without tumor implantation. The scale bar indicates 4 mm.