Figure 4

In vitro cleavage assay of BCL10 and mutants by MALT1. (A) His-tagged BCL10 and BCL10 mutants were expressed in E. coli, purified and incubated with increasing concentrations of His-tagged MALT1 or MALT1C464A mutant respectively. Samples were resolved by SDS/PAGE, followed by Western blot analysis using anti-MALT1 (top left) or anti-BCL10 antibody. Data are representative of three separate experiments. (B) Quantitative analysis of (A). The percentage of BCL10 processed was defined as the signal of cleaved product divided by the sum of signal of full-length protein and the cleaved product.