Figure 3

Induction of ICAM-1 expression in DLD-1 and SW48 cells by SDF-1 stimulation. Samples were isolated at the indicated time periods or doses. All bar graphs represent multiple increases of control cells (CL) normalized to 18S rRNA by real-time PCR analysis (A, C). The cell surface ICAM-1 protein expression levels were determined by cell surface ELISA analyses (B, D). DLD-1 and SW48 cells were stimulated with 10 ng/mL SDF-1 for the times indicated (A, B), or stimulated with SDF-1 at various doses for 4 h (C, D). Data are shown as mean ± SEM. *P < 0.05 versus CL DLD-1 cells. (E) The expression of total ICAM-1 in DLD-1 cell lysate after SDF-1 stimulation for the times indicated was determined using Western blotting. (F) Before being kept as CL, cells were pretreated with AMD3100 for 1 h and were then stimulated with SDF-1 for the times indicated. *p < 0.05 versus CL. ^#p < 0.05 versus DMSO-treated cells. (G) DLD-1 cells were stimulated with SDF-1 for the times indicated. The VCAM-1 mRNA expression level was determined by real-time PCR analysis normalized to 18S rRNA.