Figure 5

The roles of Sp1, C/EBP-β and NF-κB in SDF-1-induced ICAM-1 mRNA expression and promoter activity. The ICAM-1 promoter p537-Luc plasmid and deletion promoter constructs. DLD-1 cells were cotransfected with 5^′-deletion constructs and stimulated with 10 ng/mL SDF-1 for 4 h. ICAM-1 promoter activity was measured using a luciferase assay normalized to β-galactosidase activity and was shown to be relative to that of DLD-1 cells transfected with p537-Luc (set to 100%). *P < 0.05 versus p537-Luc.