JNK suppression is essential for 17β-Estradiol inhibits prostaglandin E2-Induced uPA and MMP-9 expressions and cell migration in human LoVo colon cancer cells
- Hsi-Hsien Hsu1, 2,
- Wei-Syun Hu3, 4,
- Yueh-Min Lin5, 6,
- Wei-Wen Kuo7,
- Li-Mien Chen8,
- Wei-Kung Chen9,
- Jin-Ming Hwang10,
- Fuu-Jen Tsai9,
- Chung-Jung Liu†12, 13 and
- Chih-Yang Huang†11, 14, 15Email author
© Hsu et al; licensee BioMed Central Ltd. 2011
Received: 5 April 2011
Accepted: 22 August 2011
Published: 22 August 2011
Epidemiological studies demonstrate that the incidence and mortality rates of colorectal cancer in women are lower than in men. However, it is unknown if 17β-estradiol treatment is sufficient to inhibit prostaglandin E2 (PGE2)-induced cellular motility in human colon cancer cells.
We analyzed the protein expression of urokinase plasminogen activator (uPA), tissue plasminogen activator (tPA), matrix metallopeptidases (MMPs), plasminogen activator inhibitor-1 (PAI-1) and tissue inhibitor of metalloproteinases (TIMPs), and the cellular motility in PGE2-stimulated human LoVo cells. 17β-Estradiol and the inhibitors including LY294002 (Akt activation inhibitor), U0126 (ERK1/2 inhibitor), SB203580 (p38 MAPK inhibitor), SP600125 (JNK1/2 inhibitor), QNZ (NFκB inhibitor) and ICI 182 780 were further used to explore the inhibitory effects of 17β-estradiol on PGE2-induced LoVo cell motility. Student's t-test was used to analyze the difference between the two groups.
Upregulation of urokinase plasminogen activator (uPA), tissue plasminogen activator (tPA) and matrix metallopeptidases (MMPs) is reported to associate with the development of cancer cell mobility, metastasis, and subsequent malignant tumor. After administration of inhibitors including LY294002, U0126, SB203580, SP600125 or QNZ, we found that PGE2 treatment up-regulated uPA and MMP-9 expression via JNK1/2 signaling pathway, thus promoting cellular motility in human LoVo cancer cells. However, PGE2 treatment showed no effects on regulating expression of tPA, MMP-2, plasminogen activator inhibitor-1 (PAI-1), tissue inhibitor of metalloproteinase-1, -2, -3 and -4 (TIMP-1, -2, -3 and -4). We further observed that 17β-estradiol treatment inhibited PGE2-induced uPA, MMP-9 and cellular motility by suppressing activation of JNK1/2 in human LoVo cancer cells.
Collectively, these results suggest that 17β-estradiol treatment significantly inhibits PGE2-induced motility of human LoVo colon cancer cells.
Colorectal carcinoma (CRC) is one of the most prevalent cancers world-wide , and is the secondary leading cause of cancer-related mortality in the developed countries . Colon cancer accounts for more than 130,000 new cases per year  and causes more than 56,000 deaths per year in United States  despite the advanced chemotherapeutic treatments.
Degradation of extracellular matrix (ECM) is closely associated the development of malignant tumor. ECM degradation by extracellular proteinases accelerates the progress of tumor cell invasion and metastasis . The proteolytic proteinase systems primarily responsible for ECM degradation in vivo are matrix metalloproteinase (MMPs) and plasminogen activator (PA) systems [5, 6]. Matrix metalloproteinases (MMPs) are a family of functionally related zinc-containing enzymes that include interstitial collagenases, gelatinases, stromelysin, matrilysin, metalloelastase, and membrane-type MMPs [7, 8]. Upregulation of MMP-2 and MMP-9 has been shown to play a key role in the progression, invasion, metastasis of colorectal cancer in animal models and patients . MMP activity is closely controlled by physiological inhibitors, TIMPs including TIMP-1, -2, -3 and -4 . Another proteolytic plasminogen system with its plasminogen activators (PA), such as urokinase-type plasminogen activators (uPA) and tissue-type plasminogen activators (tPA) is showed to activate MMPs and to be involved in colon cancer progression . Upregulation of uPA and tPA is considered as a marker of several types of malignant cancer including colon cancer .
Epidemiological studies demonstrate that the incidence and mortality rates of colorectal cancer in women are lower than in men . Estrogen (E2) performs the profound effects on target tissue is mediated by two estrogen receptor (ER) subtypes ERα and ERβ . ERα and ERβ have been identified in colon tissue in both sexes . In observational studies, estrogen exerts a protective role against the development of fatal colon cancer with a substantially decreased risk in women receiving hormone replacement therapy (HRT) [16–18], and a reduced mortality from this disease . However, the precise mechanism behind protective effects of 17β-estradiol against PGE2-induced progression in colon cancer remains unclear. In the present study, we examined the effects of 17β-estradiol on PGE2-induced cellular motility in human LoVo colon cancer cells, and further identified the precise molecular and cellular mechanisms behind this protective property. The results demonstrated that 17β-estradiol treatment inhibits PGE2-induced cellular motility and expression of uPA and MMP-9 by suppressing the activation of JNK1/2 in LoVo cells. The present study suggests that 17β-estradiol presents the properties of anti-cancer by inhibiting PGE2-induced migration in human LoVo cancer cells.
Materials and Methods
Cells, Antibodies, Reagents and Enzymes
Human colon cancer cell lines, LoVo, were obtained from the American Tissue Culture Collection (ATCC) (Rockville, MD, USA). LoVo cells were established from the metastatic nodule resected from a 56-year-old colon adenocarcinoma patient. 17β-estradiol (E2) and hydroxyurea were purchased from Sigma (Sigma Chemical Co., St. Louis, Missouri, USA). Prostaglandins E2 (PGE2) was purchased from CALBIOCHEM (Darmstadt, Germany). The LY294002 (PI3K inhibitor), U0126 (MEK1/2 inhibitor), SB203680 (p38 MAPK inhibitor), SP600125 (JNK inhibitor), and ER antagonist ICI 182,780 (ICI) were purchased from TOCRIS (Ellisville, Missouri, USA). 6-Amino-4-(4-phenoxyphenylethylamino) quinazoline (QNZ), NFκB activation inhibitor was purchased from Peptides International (Louisville, Kentucky, USA). We utilized the following antibodies against JNK1/2, phospho-JNK1/2, uPA, tPA, PAI-1, MMP-2, MMP-9, TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (Santa Cruz Biotechnology, Inc. Santa Cruz, California, USA); α-tubulin (Lab Vision Corporation, Fremont, California, USA) as loading control. Goat anti-mouse IgG antibody conjugated to horseradish peroxidase and goat anti-rabbit IgG antibody conjugated to horseradish peroxidase and rabbit anti-goat IgG horseradish peroxidase conjugate were purchased from Santa Cruz Biotechnology, Inc. in California, USA.
LoVo colon cancer cell line from the American Type Culture Collection (ATCC) (Rockville, MD) were cultured on 100-mm or 60-mm culture dishes in Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 μg/ml penicillin, 100 μg/ml streptomycin, 2 mM glutamine, 1 mM HEPS buffer, and 10% Clontech fetal bovine serum in humidified air (5% CO2) at 37°C
To isolate total proteins, cultured LoVo cells were washed with cold PBS and resuspended in lysis buffer (50 mM Tris, pH 7.5, 0.5 M NaCl, 1.0 mM EDTA, pH 7.5, 10% glycerol, 1 mM BME, 1% IGEPAL-630 and a proteinase inhibitor cocktail (Roche Molecular Biochemicals)). After incubation for 30 min on ice, the supernatant was collected by centrifugation at 12000 g for 15 min at 4°C, and the protein concentration was determined by the Bradford method. Sample containing equal proteins (60 μg) were loaded and analyzed by Western blot analysis. Briefly, proteins were separated by 12% SDS-PAGE and transferred onto PVDF membrane (Millipore, Belford, Massachusetts, USA). Membrane were blocked with blocking buffer (5% non-fat dry milk, 20 mM Tris-HCl, pH 7.6, 150 mM NaCl, and 0.1% Tween 20) for at least 1 h at room temperature. Membranes were incubated with primary antibodies in the above solution on an orbit shaker at 4°C overnight. Following primary antibody incubation, membranes were incubated with horseradish peroxidase-linked secondary antibodies (anti-rabbit, anti-mouse, or anti-goat IgG).
Migration assay was performed using the 48-well Boyden chamber (Neuro Probe) plate with the 8-μm pore size polycarbonate membrane filters . The lower compartment was filled with DMEM containing 20% FCS. LoVo cells were placed in the upper part of the Boyden chamber containing serum-free medium and incubated for 48 h. After incubation, the cells on membrane filter were fixed with methanol and stained with 0.05% Giemsa for 1 h. The cells on upper surface of the filter were removed with a cotton swab. The filters were then rinsed in double distilled water until additional stain was leached. The cells then were air-dried for 20 min. The migratory phenotypes were determined by counting the cells that migrated to the lower side of the filter with microscopy at 200× and 400× magnification, respectively. The fourth fields were counted for each filter, and each sample was assayed in triplicate.
Each experiment was duplicated at least three times. Results were presented as the mean ± SE, and statistical comparisons were made using the Student's t test. Significance was defined at the p < 0.05 or 0.01 levels.
The Effects of Prostaglandin E2 on Expression of uPA, tPA, MMP-2 and MMP-9 in Human LoVo Colon Cancer Cells
The Effects of Prostaglandin E2 on the Expression of PAI-1 and TIMPs in Human LoVo Colon Cancer Cells
JNK1/2 Mediates PGE2-Upregulated uPA and MMP-9 in Human LoVo Colon Cancer Cells
To further explore the effects of PGE2 on activation of JNK1/2 in human LoVo colon cancer cells, we treated LoVo cells with PGE2 (10-6M) for various time periods (15 min, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h), and subsequently measured the phosphorylation/activation of proteins by immunoblotting assay. Phosphorylation of JNK1/2 was significantly induced within 15 min in response to PGE2 stimulation, and was maintained up for 24 h (Figure 3B). The findings suggested that administration of PGE2 may induce the motility of human colon cancer by inducing the activation of JNK1/2.
17β-Estradiol Inhibits PGE2-Induced Expression of uPA and MMP-9 by Suppressing Activation of JNK1/2
17β-Estradiol Inhibits PGE2-Induced Cell Migration in Human LoVo Colon Cancer Cells
Upregulation of MMPs is reported to contribute to ECM remodeling, tumor cell invasion and metastasis, thus leading to the development of malignant tumor . Both mRNA levels MMP-2 and MMP-9 have been found to be overexpressed in colon carcinomas [21, 22]. In the observations of Collins et al. , MMP-2 mRNA is more significantly expressed in tumor lesions than in normal colon tissues. Immunostaining assay showed that MMP-9 expression is more frequently present in advanced tumor stages, and in invasive tumor regions wherein cancer cells are in close proximity of inflammatory cells, suggesting that locally proteolytic and collagenlytic activities contributes to the property of invasion in colorectal cancers . In the stage of cancer development, upregulation of MMP-2 and MMP-9 accelerates cell migration and invasion in colorectal cancer , thus resulting in the development of malignant tumor, poor prognosis, and shortening disease-free period and overall survival. Here, we founded that administration of 17β-estradiol significantly inhibited PGE2-induced cell migration, and downregulated PGE2-upregulated expression of MMP-9 in LoVo cells. The findings suggested that 17β-estradiol may impair PGE2-promoted cell motility by inhibiting the expression of MMP-9.
Another plasminogen activator system (PAS) with upregulation of uPA and tPA is reported to be involved in MMPs activation and colon cancer development [11, 25]. uPA was significantly greater in tumour tissues than normal tissues . uPA upregulated in colon tumor tissue enhances colorectal cancer invasion and metastasis, and this upregulation in uPA is correlated with Dukes's staging and lymphatic invasion . Upregulation in uPA and tPA expression is considered as a marker malignant colon cancer [11, 12]. In the present study, we observed that the significant reduction in protein level of uPA was observed after 17β-estradiol treatment in human colon cancer cells. These findings suggested that downregulation of uPA is involved in 17β-estradiol-mediated anticancer effects.
Activation of MMPs is regulated by physiological inhibitors TIMPs . TIMPs not only directly inhibit MMPs but also form complexes with MMPs to control activation and stability of MMPs [10, 29]. Four different TIMP species have been identified as TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP binds to MMP in a 1:1 stoichiometric ratio. Induction of functional TIMP-3 in TIMP-3-deficient human DLD-1 colon cells shows a growth arrest and inhibits tumor growth in vivo. Injection of AdTIMP-2 into preestablished tumors presents the significant reduced tumor growth rates by approximately 60-80% and tumor-associated angiogenesis index by approximately 25-75%. Metastasis of LLC tumor was inhibited by > 90%. In addition, AdTIMP-2-treated mice showed a significantly prolonged survival , which emphasizes the importance of endogenous regulation of MMPs activity by TIMPs. In the present study, we further detected the protein level of TIMPs in human LoVo cancer cells that had been exposed to PGE2, which demonstrated that PGE2 treatment shows no influence on regulating TIMP-1, TIMP-2, TIMP-3 and TIMP-4. The results suggested that PGE2 couldn't promote the motility of colon cancer by down-regulating expressions of TIMPs. In addition, the proteolytic action of uPA and tPA is controlled by plasminogen activator inhibitors 1 and 2 (PAI-1 and PAI-2) . We thus simultaneously examined the expression of PAI-1 in LoVo cells that had been treated with PGE2. However, no influence on PAI-1 expression was observed after PGE2 stimulation.
A large number of studies have been dedicated to exploring the molecular mechanisms involved in the downregulation of cancer development. Mitogen-activated protein kinases (MAPKs) include tree major subfamilies such as the extracellularly responsive kinases (ERKs), the c-Jun N-terminal kinases (JNKs), also known as stress-activated protein kinases (SAPKs), and the p38 MAPKs . Studies have showed that ERK1/2 is involved in hepatoma-derived growth factor-induced promotion of carcinogenesis of gastric epithelial cells  and in the proliferation of pancreatic stellate cells . p38 MAPK mediates TNFα-induced MMP-9 expression, thus leading to the progression of human urinary bladder cancer cells . JNK/AP-1 signaling pathway may contribute to cellular migration and invasion of prostate cancer cells . Hepatocyte growth factor-activated both Akt and JNK enhance the proteolysis and invasiveness of human nasopharyngeal cancer cells . Abnormal response of NFkB signaling pathway may contribute to the chemoresistance in acute lymphoblastic leukaemia . In the present study, we observed that JNK1/2 signaling pathway mediated expression of uPA and MMP-9 in response to PGE2, which further contributed to cellular motility of human LoVo cancer cells. Previous studies have shown that 17β-estradiol (E2) binding to estrogen receptors (ERs) can regulate tissue/cellular responses through multiple signaling pathways . In the present study, we further observed that 17β-estradiol pretreatment inhibited PGE2-induced expression of uPA, MMP-9, and cellular motility via suppressing activation of JNK1/2 in LoVo cells. It suggested that 17β-estradiol presents the anti-cancer properties by inhibiting PGE2-promoted motility in human LoVo cancer cells.
Estrogen receptor α and β (ERα and β) have been identified in colon tissue [15, 39, 40]. In the normal colon mucosa, there are no significant differences in the protein expression of ERα and β between men and women . Epidemiological data on taking home replacement therapy (HRT) suggests that the loss of estrogen inactivation may be an important mechanism in the pathogenesis of colonic cancer . Studies suggest that estrogen exerts a protective role against the development of fatal colon cancer with a substantially decreased risk in women receiving HRT [16–18], and a reduced mortality from this disease . In this study, we observed that 17β-estradiol reduces PGE2-induced motility of LoVo colon cancer cells derived from male origin. The above study findings strongly suggest that estrogen treatment is an important program against the development of colon cancer.
Estrogen has been reported to modulate the activity of the multiple signaling cascades [42, 43] such as the induction of translocation of ER to the cell membrane in MCF-7 cells , and then E2/ERs complex interact with caveolin-1/-2 in caveolae regions of the plasma membrane  wherein cavelin-1 is associated with signaling molecules, such as G proteins, growth factor receptors (IGFIR, EGFR), non-growth factor tyrosine kinase (Src, Ras), and linker proteins (MNAR, striatin) [46, 47]. In addition, membrane ER can activate Src-MMP2/9-EGFR-MAPK pathway through Gαi protein in breast cancer cells , and activate MAPK-dependent endothelial nitric oxide synthase (eNOS) to increase NO production, contributing to the beneficial effects on cardiac cells [48, 49]. In COS7 cell, cytosolic ER binds to membrane IGF-IR and rapidly activates IGF-IR-Ras-Raf-MAPKK-ERK1/2 signaling cascade, which in turn activates ER in a positive feedback loop . In the present study, we observed that 17β-estradiol (10-8 M) treatment rapidly reduces the phosphorylation of JNK1/2 within 15 min. in human LoVo colon cancer cells, which suggests that estrogen-membrane ER complex might majorly reduce PGE2-induced JNK1/2 phosphorylation through non-genomic effect. Previous studies have reported that ERβ is expressed greater in normal colon tissues than pathologic tissues , and that the increased ratio of ERα and β due to altered expression of ER subtypes is found in patients with CRC , supporting the role of ERβ as a relevant prognostic biomarker of tumor progression . According to these previous studies, we speculated that 17β-estradiol might decrease PGE2-induced cellular motility through ERβ in human LoVo colon cancer cells.
In summary, we found that PGE2 rapidly activates JNK1/2 kinase, and then increases the protein levels of uPA and MMP-9, which further promotes cellular motility in human LoVo cancer cells. 17β-Estradiol presents the properties of anti-cancer by downregulating expression of uPA and MMP-9 via deactivation of JNK1/2 in LoVo cells. These results also demonstrate that 17β-estradiol efficiently inhibit PGE2-induced LoVo cell motility. These findings might explain that why the incidence and mortality rates of colorectal cancer in women are lower than in men; and that estrogen exerts a protective role against the development of fatal colon cancer with a substantially decreased risk in women receiving hormone replacement therapy (HRT) and a reduced mortality from this disease.
extracellular signal regulated kinase
- p38 MAPK:
p38 mitogen-activated protein kinase
c-Jun N-terminal kinase
nuclear factor κ B
protein kinase B
urokinase plasminogen activator
tissue plasminogen activator
plasminogen activator inhibitor-1
tissue inhibitor of metalloproteinases
Dulbecco's modified Eagle's medium
glyceraldehyde-3- phosphate dehydrogenase
- Jemal A, Murray T, Ward E: Cancer statistics, 2005. CA Cancer J Clin. 2005, 55: 10-30. 10.3322/canjclin.55.1.10.View ArticlePubMedGoogle Scholar
- Greenwald P: Colon cancer overview. Cancer. 1992, 70: 1206-1215. 10.1002/1097-0142(19920901)70:3+<1206::AID-CNCR2820701504>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Jemal A, Thomas A, Murray T: Cancer statistics, 2002. CA Cancer J Clin. 2002, 52: 23-47. 10.3322/canjclin.52.1.23.View ArticlePubMedGoogle Scholar
- Jemal A, Tiwari RC, Murray T: Cancer statistics, 2004. CA Cancer J Clin. 2004, 54: 8-29. 10.3322/canjclin.54.1.8.View ArticlePubMedGoogle Scholar
- Liotta LA, Stetler-Stevenson WG: Tumor invasion and metastasis: an imbalance of positive and negative regulation. Cancer Res. 1991, 51: 5054s-5059s.PubMedGoogle Scholar
- Yoon WH, Jung YJ, Kim TD: Gabexate mesilate inhibits colon cancer growth, invasion, and metastasis by reducing matrix metalloproteinases and angiogenesis. Clin Cancer Res. 2004, 10 (13): 4517-4526. 10.1158/1078-0432.CCR-04-0084.View ArticlePubMedGoogle Scholar
- Coussens LM, Fingleton B, Matrisian LM: Matrix metalloproteinase inhibitors and cancer: trials and tribulations. Science. 2002, 295 (5564): 2387-2392. 10.1126/science.1067100.View ArticlePubMedGoogle Scholar
- Ellerbroek SM, Stack MS: Membrane associated matrix metalloproteinases in metastasis. Bioessays. 1999, 21 (11): 940-949. 10.1002/(SICI)1521-1878(199911)21:11<940::AID-BIES6>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Mook OR, Frederiks WM, Van Noorden CJ: The role of gelatinases in colorectal cancer progression and metastasis. Biochim Biophys Acta. 2004, 1705 (2): 69-89.PubMedGoogle Scholar
- Gomez DE, Alonso DF, Yoshiji H: Tissue inhibitors of metalloproteinases: structure, regulation and biological functions. Eur J Cell Biol. 1997, 74 (2): 111-122.PubMedGoogle Scholar
- Berger DH: Plasmin/plasminogen system in colorectal cancer. World J Surg. 2002, 26 (7): 767-771. 10.1007/s00268-002-4050-8.View ArticlePubMedGoogle Scholar
- Seetoo DQ, Crowe PJ, Russell PJ: Quantitative expression of protein markers of plasminogen activation system in prognosis of colorectal cancer. J Surg Oncol. 2003, 82 (3): 184-193. 10.1002/jso.10210.View ArticlePubMedGoogle Scholar
- Jacobs ET, Thompson PA, Martinez ME: Diet, gender, and colorectal neoplasia. J Clin Gastroenterol. 2007, 41 (8): 731-746. 10.1097/MCG.0b013e3180338e56.View ArticlePubMedGoogle Scholar
- Katzenellenbogen JA, Katzenellenbogen BS: Nuclear hormone receptors: ligand-activated regulators of transcription and diverse cell responses. Chem Biol. 1996, 3 (7): 529-536. 10.1016/S1074-5521(96)90143-X.View ArticlePubMedGoogle Scholar
- Campbell-Thompson M, Lynch IJ, Bhardwaj B: Expression of estrogen receptor (ER) subtypes and ERbeta isoforms in colon cancer. Cancer Res. 2001, 61 (2): 632-640.PubMedGoogle Scholar
- Gambacciani M, Monteleone P, Sacco A: Hormone replacement therapy and endometrial, ovarian and colorectal cancer. Best Pract Res Clin Endocrinol Metab. 2003, 17 (1): 139-147. 10.1016/S1521-690X(02)00086-6.View ArticlePubMedGoogle Scholar
- Grodstein F, Martinez ME, Platz EA: Postmenopausal hormone use and risk for colorectal cancer and adenoma. Ann Intern Med. 1998, 128 (9): 705-712.View ArticlePubMedGoogle Scholar
- Chlebowski RT, Wactawski-Wende J, Ritenbaugh C: Estrogen plus progestin and colorectal cancer in postmenopausal women. N Engl J Med. 2004, 350 (10): 991-1004. 10.1056/NEJMoa032071.View ArticlePubMedGoogle Scholar
- Calle EE, Miracle-McMahill HL, Thun MJ: Estrogen replacement therapy and risk of fatal colon cancer in a prospective cohort of postmenopausal women. J Natl Cancer Inst. 1995, 87 (7): 517-523. 10.1093/jnci/87.7.517.View ArticlePubMedGoogle Scholar
- Lai TY, Chen LM, Lin JY: 17beta-estradiol inhibits prostaglandin E2-induced COX-2 expressions and cell migration by suppressing Akt and ERK1/2 signaling pathways in human LoVo colon cancer cells. Mol Cell Biochem. 2010, 342 (1-2): 63-70. 10.1007/s11010-010-0469-7.View ArticlePubMedGoogle Scholar
- Chan CC, Menges M, Orzechowski HD: Increased matrix metalloproteinase 2 concentration and transcript expression in advanced colorectal carcinomas. Int J Colorectal Dis. 2001, 16 (3): 133-140. 10.1007/s003840100287.View ArticlePubMedGoogle Scholar
- Roeb E, Dietrich CG, Winograd R: Activity and cellular origin of gelatinases in patients with colon and rectal carcinoma differential activity of matrix metalloproteinase-9. Cancer. 2001, 92 (10): 2680-2691. 10.1002/1097-0142(20011115)92:10<2680::AID-CNCR1622>3.0.CO;2-7.View ArticlePubMedGoogle Scholar
- Collins HM, Morris TM, Watson SA: Spectrum of matrix metalloproteinase expression in primary and metastatic colon cancer: relationship to the tissue inhibitors of metalloproteinases and membrane type-1-matrix metalloproteinase. Br J Cancer. 2001, 84 (12): 1664-1670. 10.1054/bjoc.2001.1831.PubMed CentralView ArticlePubMedGoogle Scholar
- Jeziorska M, Haboubi NY, Schofield PF: Distribution of gelatinase B (MMP-9) and type IV collagen in colorectal carcinoma. Int J Colorectal Dis. 1994, 9 (3): 141-148. 10.1007/BF00290191.View ArticlePubMedGoogle Scholar
- Zucker S, Vacirca J: Role of matrix metalloproteinases (MMPs) in colorectal cancer. Cancer Metastasis Rev. 2004, 23 (1-2): 101-117.View ArticlePubMedGoogle Scholar
- Baker EA, Leaper DJ: The plasminogen activator and matrix metalloproteinase systems in colorectal cancer: relationship to tumour pathology. Eur J Cancer. 2003, 39 (7): 981-988. 10.1016/S0959-8049(03)00065-0.View ArticlePubMedGoogle Scholar
- Baker EA, Bergin FG, Leaper DJ: Plasminogen activator system, vascular endothelial growth factor, and colorectal cancer progression. Mol Pathol. 2000, 53 (6): 307-312. 10.1136/mp.53.6.307.PubMed CentralView ArticlePubMedGoogle Scholar
- Li YY, McTiernan CF, Feldman AM: Interplay of matrix metalloproteinases, tissue inhibitors of metalloproteinases and their regulators in cardiac matrix remodeling. Cardiovasc Res. 2000, 46 (2): 214-224. 10.1016/S0008-6363(00)00003-1.View ArticlePubMedGoogle Scholar
- Goldberg GI, Marmer BL, Grant GA: Human 72-kilodalton type IV collagenase forms a complex with a tissue inhibitor of metalloproteases designated TIMP-2. Proc Natl Acad Sci USA. 1989, 86 (21): 8207-8211. 10.1073/pnas.86.21.8207.PubMed CentralView ArticlePubMedGoogle Scholar
- Bian J, Wang Y, Smith MR: Suppression of in vivo tumor growth and induction of suspension cell death by tissue inhibitor of metalloproteinases (TIMP)-3. Carcinogenesis. 1996, 17 (9): 1805-1811. 10.1093/carcin/17.9.1805.View ArticlePubMedGoogle Scholar
- Li H, Lindenmeyer F, Grenet C: AdTIMP-2 inhibits tumor growth, angiogenesis, and metastasis, and prolongs survival in mice. Hum Gene Ther. 2001, 12 (5): 515-526. 10.1089/104303401300042429.View ArticlePubMedGoogle Scholar
- Marino M, Galluzzo P, Ascenzi P: Estrogen signaling multiple pathways to impact gene transcription. Curr Genomics. 2006, 7 (8): 497-508. 10.2174/138920206779315737.PubMed CentralView ArticlePubMedGoogle Scholar
- Mao J, Xu Z, Fang Y: Hepatoma-derived growth factor involved in the carcinogenesis of gastric epithelial cells through promotion of cell proliferation by Erk1/2 activation. Cancer Sci. 2008, 99 (11): 2120-2127. 10.1111/j.1349-7006.2008.00928.x.View ArticlePubMedGoogle Scholar
- Schwer CI, Guerrero AM, Humar M: Heme oxygenase-1 inhibits the proliferation of pancreatic stellate cells by repression of the ERK1/2 pathway. J Pharmacol Exp Ther. 2008, 327 (3): 863-871. 10.1124/jpet.108.136549.View ArticlePubMedGoogle Scholar
- Lee SJ, Park SS, Lee US: Signaling pathway for TNF-alpha-induced MMP-9 expression: Mediation through p38 MAP kinase, and inhibition by anti-cancer molecule magnolol in human urinary bladder cancer 5637 cells. Int Immunopharmacol. 2008, 8 (13-14): 1821-1826. 10.1016/j.intimp.2008.08.018.View ArticlePubMedGoogle Scholar
- Kwon GT, Cho HJ, Chung WY: Isoliquiritigenin inhibits migration and invasion of prostate cancer cells: possible mediation by decreased JNK/AP-1 signaling. J Nutr Biochem. 2009, 20 (9): 663-676. 10.1016/j.jnutbio.2008.06.005.View ArticlePubMedGoogle Scholar
- Zhou HY, Wan KF, Ip CK: Hepatocyte growth factor enhances proteolysis and invasiveness of human nasopharyngeal cancer cells through activation of PI3K and JNK. FEBS Lett. 2008, 582 (23-24): 3415-3422. 10.1016/j.febslet.2008.09.004.View ArticlePubMedGoogle Scholar
- Stankovic T, Marston E: Molecular mechanisms involved in chemoresistance in paediatric acute lymphoblastic leukaemia. Srp Arh Celok Lek. 2008, 136 (3-4): 187-192. 10.2298/SARH0804187S.View ArticlePubMedGoogle Scholar
- Mostafaie N, Kallay E, Sauerzapf E: Correlated downregulation of estrogen receptor beta and the circadian clock gene Per1 in human colorectal cancer. Mol Carcinog. 2009, 48 (7): 642-647. 10.1002/mc.20510.View ArticlePubMedGoogle Scholar
- Nussler NC, Reinbacher K, Shanny N: Sex-specific differences in the expression levels of estrogen receptor subtypes in colorectal cancer. Gend Med. 2008, 5 (3): 209-217. 10.1016/j.genm.2008.07.005.View ArticlePubMedGoogle Scholar
- English MA, Kane KF, Cruickshank N: Loss of estrogen inactivation in colonic cancer. J Clin Endocrinol Metab. 1999, 84 (6): 2080-2085. 10.1210/jc.84.6.2080.View ArticlePubMedGoogle Scholar
- Yang XP, Reckelhoff JF: Estrogen, hormonal replacement therapy and cardiovascular disease. Curr Opin Nephrol Hypertens. 2011, 20: 133-138. 10.1097/MNH.0b013e3283431921.PubMed CentralView ArticlePubMedGoogle Scholar
- Liu CJ, Lo JF, Kuo CH: Akt mediates 17beta-estradiol and/or estrogen receptor-alpha inhibition of LPS-induced tumor necresis factor-alpha expression and myocardial cell apoptosis by suppressing the JNK1/2-NFkappaB pathway. J Cell Mol Med. 2009, 13 (9B): 3655-3667. 10.1111/j.1582-4934.2009.00669.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Cheskis BJ, Greger JG, Nagpal S: Signaling by estrogens. J Cell Physiol. 2007, 213 (3): 610-617. 10.1002/jcp.21253.View ArticlePubMedGoogle Scholar
- Razandi M, Alton G, Pedram A: Identification of a structural determinant necessary for the localization and function of estrogen receptor alpha at the plasma membrane. Mol Cell Biol. 2003, 23 (5): 1633-1646. 10.1128/MCB.23.5.1633-1646.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Hammes SR, Levin ER: Extranuclear steroid receptors: nature and actions. Endocr Rev. 2007, 28 (7): 726-741. 10.1210/er.2007-0022.View ArticlePubMedGoogle Scholar
- Razandi M, Pedram A, Park ST: Proximal events in signaling by plasma membrane estrogen receptors. J Biol Chem. 2003, 278 (4): 2701-2712. 10.1074/jbc.M205692200.View ArticlePubMedGoogle Scholar
- Chen Z, Yuhanna IS, Galcheva-Gargova Z: Estrogen receptor alpha mediates the nongenomic activation of endothelial nitric oxide synthase by estrogen. J Clin Invest. 1999, 103 (3): 401-406. 10.1172/JCI5347.PubMed CentralView ArticlePubMedGoogle Scholar
- Mendelsohn ME, Karas RH: The protective effects of estrogen on the cardiovascular system. N Engl J Med. 1999, 340: 1801-1811. 10.1056/NEJM199906103402306.View ArticlePubMedGoogle Scholar
- Kahlert S, Nuedling S, van Eickels M: Estrogen receptor alpha rapidly activates the IGF-1 receptor pathway. J Biol Chem. 2000, 275: 18447-18453. 10.1074/jbc.M910345199.View ArticlePubMedGoogle Scholar
- Castiglione F, Taddei A, Degl'Innocenti DR: Expression of estrogen receptor beta in colon cancer progression. Diagn Mol Pathol. 2008, 17 (4): 231-236. 10.1097/PDM.0b013e3181656d67.View ArticlePubMedGoogle Scholar
- Barone M, Scavo MP, Papagni S: ERbeta expression in normal, adenomatous and carcinomatous tissues of patients with familial adenomatous polyposis. Scand J Gastroenterol. 2010, 45 (11): 1320-1328. 10.3109/00365521.2010.487915.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.