CXC chemokine ligand 12/Stromal cell-derived factor-1 regulates cell adhesion in human colon cancer cells by induction of intercellular adhesion molecule-1
© Tung et al.; licensee BioMed Central Ltd. 2012
Received: 30 July 2012
Accepted: 22 October 2012
Published: 25 October 2012
The CXC chemokine ligand 12 (CXCL12)/stromal cell-derived factor-1 (SDF-1) and CXC receptor 4 (CXCR4) axis is involved in human colorectal cancer (CRC) carcinogenesis and can promote the progression of CRC. Interaction between CRC cells and endothelium is a key event in tumor progression. The aim of this study was to investigate the effect of SDF-1 on the adhesion of CRC cells.
Human CRC DLD-1 cells were used to study the effect of SDF-1 on intercellular adhesion molecule-1 (ICAM-1) expression and cell adhesion to endothelium.
SDF-1 treatment induced adhesion of DLD-1 cells to the endothelium and increased the expression level of the ICAM-1. Inhibition of ICAM-1 by small interfering RNA (siRNA) and neutralizing antibody inhibited SDF-1-induced cell adhesion. By using specific inhibitors and short hairpin RNA (shRNA), we demonstrated that the activation of ERK, JNK and p38 pathways is critical for SDF-1-induced ICAM-1 expression and cell adhesion. Promoter activity and transcription factor ELISA assays showed that SDF-1 increased Sp1-, C/EBP-β- and NF-κB-DNA binding activities in DLD-1 cells. Inhibition of Sp1, C/EBP-β and NF-κB activations by specific siRNA blocked the SDF-1-induced ICAM-1 promoter activity and expression. The effect of SDF-1 on cell adhesion was mediated by the CXCR4.
Our findings support the hypothesis that ICAM-1 up-regulation stimulated by SDF-1 may play an active role in CRC cell adhesion.
KeywordsColorectal cancer Stromal cell-derived factor-1 Intercellular adhesion molecule-1 Cell adhesion Transcriptional regulation
Colorectal cancer (CRC) is one of the most common malignancies and is the fourth leading cause of cancer death worldwide. Death due to colorectal cancer usually results from metastatic spread of cancer cells . Chemokines play a pivotal role in tumor progression because they may affect growth, adhesion and metastasis of tumor cells [2, 3]. CXC chemokine ligand 12 (CXCL12)/Stromal cell-derived factor (SDF-1) is a member of the CXC chemokine family, which plays an important role in chemotaxis, haematopoiesis, angiogenesis and tumor spread and metastasis [4, 5]. SDF-1 is expressed in several cancer cells and is involved in tumor cell migration and metastasis [6, 7]. Several studies have indicated that CRC cells promoted their survival and migration to distant tissues by interaction between SDF-1 and its specific receptor, CXC receptor 4 (CXCR4). The higher expression of CXCR4 in tumor tissue correlates with poor prognosis and poor survival in CRC patients [8, 9].
Tumor cell adhesion is not only associated in tumor cell detachment from the primary carcinoma, but is also involved in cell attachment to distant tissue . The maintenance and promotion of cell adhesion are particularly important processes in CRC progression and metastasis. The intercellular adhesion molecule-1 (ICAM-1) is a member of the immunoglobulin (Ig) gene superfamily of adhesion molecules. It is involved in cancer cell adhesion and in the immune responses of tumors [11, 12]. The interaction between ICAM-1 and its specific ligand may facilitate adhesion of cancer cells to the vascular endothelium and subsequently in the promotion of metastasis. It has been reported that the serum level of soluble (s)ICAM-1 is elevated in patients with cancers . High serum levels of sICAM-1 have been shown to be correlated with tumor progression in cases of CRC and associated with liver metastasis [14, 15]. Normal colon tissues do not express ICAM-1, but this adhesion molecule can be up-regulated on malignant transformation . Several studies also suggest that ICAM-1 plays an important role in CRC progression and metastasis [17–19]. It has been demonstrated that SDF-1 effectively induced the expression of urokinase plasminogen activator in CRC cells . In addition, SDF-1 has been implicated in integrin-mediated adhesion in lung cancer and renal cancer cells [21–23]. SDF-1–mediated cell adhesion via ICAM-1 has also been reported in leukemia cells . Firm cell adhesion between CD34+ cells and endothelial cells is reported to occur when ICAM-1 and SDF-1 are co-immobilized . However, it is not clear whether the regulation of ICAM-1 expression and cell adhesion are mediated by SDF-1 in colorectal cancer cells.
Mitogen-activated protein kinases (MAPKs) are known as major signaling pathways that play an essential role in the development of inflammation and cancer . Furthermore, they have been reported to be involved in cancer cell pathological functions, including invasion and angiogenesis . In the present study, we show that SDF-1 induces ICAM-1 expression in colorectal cancer DLD-1 cells. Our data indicate that the SDF-1-induced increase of ICAM-1 expression and cell adhesion is mediated through the ERK, JNK and p38 intracellular signaling cascades, and the transcription factors Sp1, C/EBP-β and NF-κB. Our findings provide evidence of the molecular mechanisms by which SDF-1 induces ICAM-1 expression in colorectal cancer cells.
All culture materials were purchased from Gibco (Grand Island, NY, USA). PD98059 (ERK inhibitor), SP600125 (JNK inhibitor), and SB203580 (p38 inhibitor) were purchased from Calbiochem (La Jolla, CA). Polyclonal anti-ICAM-1 and anti-CXCR4 neutralizing antibody were obtained from R & D Systems (Minneapolis, MN). Mouse monoclonal antibodies (mABs) against ERK2, JNK1, phospho-ERK1/2, phospho-JNK1/2, NF-κB p65, Sp1, and C/EBP-β were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Rabbit polyclonal antibodies against p38 and mouse monoclonal phospho-p38 antibody were purchased from Cell Signaling Technology (Beverly, MA). ERK, JNK, and p38 shRNA and control shRNA (scrambled negative control containing random DNA sequences) were purchased from the National RNAi Core Facility in Academic Sinica, Taipei, Taiwan. The NF-κB p65, Sp1, and C/EBP-β-siRNA and control siRNA (scrambled negative control containing random DNA sequences) were purchased from Invitrogen (Carlsbad, CA). AMD3100 (CXCR4 inhibitor) and all other chemicals of a reagent grade were obtained from Sigma (St. Louis, MO).
The colon cancer DLD-1 and SW48 cell lines were purchased from the Bioresources Collection and Research Center (BCRC) of the Food Industry Research and Development Institute (Hsinchu, Taiwan). Cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a CO2 incubator at 37°C.
Human umbilical vein endothelial cells (HUVECs) were isolated by collagenase digestion. Briefly, human umbilical cords were washed, filled with 0.1% collagenase, and incubated at 37°C for 10 min. The suspension of ECs collected was centrifuged for 10 min at 1200 × g. The pellet was resuspended in M199 supplemented with penicillin/streptomycin and 20% FBS, after which the cells were plated onto cell culture dishes. Cultures were maintained at 37°C in a humidified atmosphere containing 5% CO2. HUVECs of passage 2 were used for experiments.
Flow cytometry analysis
The expressions of CXCR4 and ICAM-1 on the surface of DLD-1 cells were measured by indirect immunofluorescence using flow cytometry and the mouse monoclonal antibody (20 μg/mL; R&D) against CXCR4 and ICAM-1 .
Cell adhesion assay
Adherence of DLD-1 and SW48 cells to HUVECs was determined as described previously . Before the adhesion experiments, cells were cultured in low-serum medium (0.5% FBS) for 12 h and then treated with SDF-1 (10 ng/mL) for 4 h and labeled with 1, 10-dioctadecyl-3, 3, 30, 30-tetramethylindocarbocyanine (DiI; Molecular Probes, Eugene, OR) for 20 min. The labeled cells (2 × 105 cells/mL) were added to HUVECs and incubated for 1 h. In parallel experiments, DLD-1 cells were treated with specific inhibitors or transfected with specific shRNAs during SDF-1 stimulation. Non-adherent cells were removed by washing with PBS. The adherent cancer cells on the EC surface were identified and counted in 10 randomly selected microscopic fields (1.37 mm × 1.07 mm) under a Nikon Ti-E inverted epifluorescence microscope with ×10 objective, and the adhesion was expressed as a multiple compared to the controls. To determine the role of CXCR4 and ICAM-1 in the adhesion of DLD-1 and SW48 cells to HUVECs, CRC cells were pre-incubated with 20 μg/mL of anti-human CXCR4 and ICAM-1 antibodies (R&D) for 30 min, pretreated with AMD3100, or transfected with the ICAM-1 siRNA prior to the adhesion assay.
Real-time quantitative PCR
Real-time PCR of three transcripts was performed using an ABI Prism 7900HT with the FastStart DNA SYBR Green I kit (Roche). The designed primers in this study were: ICAM-1 forward primer, 5′- GTGAC ATGCA GCACC TCCTG-3′; ICAM-1 reverse primer, 5′- TCCAT GGTGA TCTCT CCTCA-3′; 18S rRNA forward primer, 5′-CGGCG ACGAC CCATT CGAAC-3′, 18S rRNA reverse primer, 5′-GAATC GAACC CTGAT TCCCC GTC-3′. Quantification was performed using the 2−ΔΔCt method . All samples were measured in duplicate. The average value of both duplicates was used as the quantitative value.
ELISA for cell surface ICAM-1 expression
ICAM-1 expression on the cancer cell surface was measured by cell surface ELISA as previously described . Briefly, DLD-1 cells cultured in 96-well plates were fixed by 4% paraformaldehyde. Cell surface ICAM-1 expression was assessed using the mouse anti-human ICAM-1 mAb, followed by a horseradish-peroxidase-conjugated secondary antibody. The absorbance of each well was measured at 490 nm after the reactions were stopped.
Western blot analysis
Samples were lysed with a buffer containing 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and a protease inhibitor mixture (PMSF, aprotinin and sodium orthovanadate). Protein concentration was determined using the Bio-Rad protein assay kit (Bio-Rad, Hercules, CA). Equal amounts of total proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) (10% running, 4% stacking), transferred onto a nitrocellulose membrane, and analyzed using the designated antibodies and the Western-Light chemiluminescent detection system (Bio-Rad).
shRNA and siRNA transfection
For shRNA transfection, DLD-1 cells were transfected with the designated MAPK shRNA plasmids by using shRNA plasmid transfection reagent (Santa Cruz). For siRNA transfection, DLD-1 cells were transfected with the specific NF-κB p65, Sp1-1, and C/EBP-β-siRNA or control siRNA by using an RNAiMAX transfection kit (Invitrogen).
Human ICAM-1 promoter constructs containing −537/+16, -413/+16, -323/+16, -245/+16, -134/+16, and −88/+16 of ICAM-1 5'-flanking DNA linked to the firefly luciferase reporter gene of plasmid pGL4 (Promega, Madison, WI) were used. DNA plasmids at a concentration of 1 mg/ml were transfected into DLD-1 cells by Lipofectamine (Gibco). The pSV-β-galactosidase plasmid was cotransfected to normalize the transfection efficiency. Values obtained were normalized to the levels of β-galactosidase in the cell lysates. β-galactosidase activities were determined with an assay kit and exhibited <20% variation between samples.
Transcription factor assays (TF ELISA assays)
Nuclear extracts of cells were prepared by nuclear protein extract kits (Panomics, Redwood City, CA). Equal amounts of nuclear proteins were used for quantitative measurements of NF-κB p65, Sp1 and C/EPB-β activation using commercially available ELISA kits (Panomics).
Chromatin immunoprecipitation assay (ChIP)
After cross-linking with 1% formaldehyde, the cells were centrifuged and then resuspended in a lysis buffer for three times of sonication of 15 sec each. Supernatants were recovered by centrifugation. Aliquots of the precleared sheared chromatin were then immunoprecipitated using 2 μg antibodies against IgG, NF-κB p65, Sp1 and C/EPB-β. The resulting DNA was used for PCR analysis, and the amplified DNA fragments were visualized on an agarose gel. The human ICAM-1 promoter region −305/-91 was amplified with the PCR primer pairs 5′-ACCTT AGCGC GGTGT AGACC-3′ and 5′-CTCCG GAACA AATGC TGC-3′.
The results are expressed as the mean ± standard error of the mean (SEM). Statistical analysis was determined using an independent Student t-test for two groups of data and analysis of variance (ANOVA) followed by Scheffe’s test for multiple comparisons. P values less than 0.05 were considered significant.
Effect of SDF-1 on adhesion of CRC cells to HUVECs
Pretreatment with AMD3100 could also inhibit adhesion of DLD-1 cells treated with different doses of SDF-1 (Figure 1C). The effect of SDF-1 on CXCR4 expression in DLD-1 cells was evaluated by flow cytometry assay using CXCR4 antibody. As shown in Figure 1D, CXCR4 is hardly expressed in DLD-1 cells, but cell surface expression is up-regulated by stimulation with SDF-1 for 4 h in DLD-1 cells.
Effect of SDF-1-induced ICAM-1 expression on adhesion of DLD-1 cells to HUVECs
Pretreatment with AMD3100 could also inhibit ICAM-1 expression of DLD-1 cells treated with different doses of SDF-1 (Figure 2C). The basal level of ICAM-1 expression in DLD-1 cells was evaluated by flow cytometry assay, as shown in Figure 2D.
SDF-1-induced ICAM-1 expression in DLD-1 and SW48 cells is dose- and time-dependent
ICAM-1 expression in the indicated times was inhibited by DLD-1 cells treated with AMD3100 (Figure 3F). In addition, SDF-1 induced only slight increases in the DLD-1 VCAM-1 transcript level compared with untreated cells (Figure 3G).
Effect of MAPK inhibitors on ICAM-1 mRNA expression and cell adhesion in DLD-1 cells
Our previous study demonstrated that the phosphorylation level of p38 in DLD-1 cells increased rapidly after stimulation by SDF-1 . In the present study, the phosphorylation of ERK, JNK and p38 in DLD-1 cells also increased after SDF-1 stimulation (Figure 4E). In addition, the SDF-1–induced phosphorylation of ERK, JNK and p38 in DLD-1 cells was inhibited by cells pretreated with AMD3100 (Figure 4E).
Sp1, C/EBP and NF-κB are essential for the SDF-1-induction of human ICAM-1 expression
We further tested whether Sp1, C/EBP-β, and NF-κB activation are involved in the signal transduction pathway leading to the SDF-1 induction of ICAM-1promoter activity. DLD-1 cells were transfected with siRNAs for Sp1, C/EBP-β, or p65 followed by stimulation with SDF-1 for 4 h. The Sp1, C/EBP-β, and NF-κB p65-specific siRNAs (compared with control siRNA) caused an 80% reduction in Sp1, C/EBP-β, and NF-κB p65 protein expressions, respectively (Figure 6D). The SDF-1-induced ICAM-1 p537-Luc promoter activity levels (Figure 6E) were significantly down-regulated by the inhibition of specific siRNAs, indicating that Sp1, C/EBP-β, and p65 are involved in the regulation of ICAM-1 gene expression. These results were confirmed by ChIP analysis. Immunoprecipitated chromosomal DNA with Sp1, C/EBP-β and p65 antibodies was subjected to PCR, using primers designed to amplify the ICAM-1 promoter region harboring these transcription factor binding sites. Sp1, C/EBP-β and NF-κB were indeed found to bind the ICAM-1 promoter region (Figure 6F).
MAPK signaling pathways were involved in SDF-1-induced ICAM-1 promoter activity
To explore whether MAPKs activate the promoter leading to ICAM-1 transcription via activations of Sp1, C/EBP-β, and NF-κB, DLD-1 cells were pretreated with MAPK inhibitors or transfected with MAPK shRNAs followed by the SDF-1 treatment. The Sp1, C/EBP-β, and NF-κB p65 activations were assessed using TF ELISA kits. Pretreatment of cells with PD98059, SP600125 and SB203580, or transfected with ERK-, JNK-, and p38-shRNA significantly inhibited the SDF-1-induced Sp1- (Figure 7B) and NF-κB p65-DNA (Figure 7D) binding activity, whereas pretreatment of cells with PD98059 and SB203580, or transfected with ERK- and p38-shRNA markedly inhibited the SDF-1-induced C/EBP-β-DNA binding activity (Figure 7C). In addition, as shown in Figure 7E, SDF-1 also induced degradation of IκB protein in DLD-1 cells in a time-dependent manner.
The cell adhesion process plays a key role in tumor progression. Several adhesion molecules, including ICAM-1, have been implicated in the tumor transformation and metastasis via the mediating adhesion of cancer cells to the vascular endothelium. This is an important procedure for executing the subsequent steps of invasion and survival of cancer cells . It has been shown that CRC metastases are related to the ability of cancer cells to adhere to the microvascular endothelium of the lung that expresses the ligands for ICAM-1 on the tumor cell surface . Previous studies demonstrated that ICAM-1 is expressed in CRC cells, and patients with higher ICAM-1 levels in plasma reveal poor prognosis compared to healthy controls [14, 17, 33]. In addition, up-regulation of ICAM-1 expression is correlated with the cancer stage and distant metastasis, suggesting that ICAM-1 plays a central role in tumor progression and metastasis . The findings of the present study suggest that the expression of ICAM-1 in DLD-1 cells may enhance their adhesiveness to HUVECs: 1) ICAM-1 was up-regulated by SDF-1 stimulation in DLD-1 cells; 2) functional blockade of ICAM-1 by siRNA and neutralizing antibody resulted in markedly decreased adherence of DLD-1 cells to HUVECs.
The expression of SDF-1 has been detected in several cancer cells, indicating that these pathways may be important in the metastasis of tumor cells [35, 36]. SDF-1 is a constitutively expressed and inducible chemokine that modulates biological and physiological processes, including embryonic development and organ homeostasis . Abnormal expression of SDF-1 has been detected in various solid tumor tissues such as ovarian cancer , breast cancer  and colorectal cancer . SDF-1 has been shown to promote the growth, invasion and metastasis of CRC cells , implying that SDF-1 plays a role in the progression of CRC; however, the mechanisms of how SDF-1 stimulates metastasis of CRC cells are not totally understood. In this study, we evaluated the molecular mechanisms underlying the roles of SDF-1 in modulating ICAM-1 expression and cell adhesion to HUVECs in colon cancer cells. Our results have demonstrated that SDF-1-induced expression of ICAM-1 expression is mediated by the MAPKs, and downstream Sp1, C/EBP-β and NF-κB signaling pathways.
The promoter region of the ICAM-1 gene has different binding sites for various transcriptional factors . Previous studies have shown that the transcription factors NF-κB, Sp1 and C/EBP-β can be activated through the MAPK pathways in colorectal cancer cells [20, 43, 44]. Regulation of gene expression through the use of combinations of different transcription factors has been observed [45, 46]. It has been reported that NF-κB and Sp1 can be activated by MAPK family members, depending on the target gene in different cells . Patel et al. reported that MAPKs mediate NF-κB and C/EBP-β activation, which leads to the transcription of specific genes . To evaluate the signaling factors upstream of the activation of NF-κB, Sp1 and C/EBP-β that lead to ICAM-1 transcriptional activation in DLD-1 cells, we investigated the role of MAPKs using specific inhibitors. In addition, we performed TF ELISA assays to demonstrate that the regulation of ICAM-1 gene expression in DLD-1 cells was mediated by increased NF-κB p65, Sp1- and C/EBP-β-DNA binding activities. Based on our results, we propose a possible signal transduction pathway in DLD-1 cells in which SDF-1 induces ERK, JNK and p38 phosphorylation, which activates NF-κB, Sp1 and C/EBP-β to lead to their binding to respective sites in the ICAM-1 promoter, thus resulting in ICAM-1 transcriptional activation.
Several studies have indicated that CRC cells express CXCR4 and CXCR7, the receptors for SDF-1, and that their survival and migration to distant tissues are promoted by SDF-1 [41, 49]. The binding of SDF-1 to CXCR4 may affect several signaling pathways, which can lead to multiple responses. Our previous study revealed that the expression of urokinase plasminogen activator in CRC DLD-1 cells by SDF-1 stimulation is mediated by CXCR4 . In order to analyze the role of CXCR4 in ICAM-1 expression in human CRC cells, we evaluated the effect of CXCR4 function-blocking antibody on SDF-1–induced ICAM-1 expression. Our results suggest that when DLD-1 cells encounter an external stimulus of SDF-1, SDF-1 induces ICAM-1 expression via CXCR4 up-regulation. Interestingly, DLD-1 cells did not express surface CXCR4 , but they had abundant CXCR4 after SDF-1 stimulation, as determined by flow cytometry. Up-regulation of CXCR4 cells is functionally important, as SDF-1-stimulated DLD-1 cells in contrast to control cells respond to SDF-1 by inducing the ICAM-1 expression and adhering to HUVECs in response to SDF-1. Previously it had been reported that CXCR4 surface expression is hardly detectable on neutrophils, but expression became apparent gradually after incubation . An alternative possibility described recently postulates that CXCR7 may serve as a co-receptor for CXCR4 and mediate SDF-1–induced intracellular signaling . Several studies have indicated that CRC cells also express CXCR7, the receptors for SDF-1, and that their survival and migration to distant tissues is promoted by SDF-1 . However, the mechanisms of CXCR4 expression in DLD-1 cells and the interaction between CXCR7 remains unknown, and further study is required to identify whether SDF-1 affects CXCR7 function and CXCR4 expression in CRC cells.
ICAM-1 expression modulates several cell functions and processes that regulate the progression of malignancies, including apoptosis, cell motility, invasion and angiogenesis. It has been shown that ICAM-1 may support mesothelial adhesion of CRC cells . Interaction of ICAM-1 on vascular or lymphatic endothelial cells has been found to promote the adhesion of lung cancer and breast cancer cells [54, 55]. In addition, ICAM-1 may be expressed by cancer cells themselves and contribute to their invasiveness .
In summary, our data contribute new information about the mechanisms by which SDF-1 induces ICAM-1 expression and cell adhesion to the endothelium in colon cancer cells.
CXC chemokine ligand 12
Stromal cell-derived factor
CXC receptor 4
Intercellular adhesion molecule-1
Mitogen-activated protein kinases
Human umbilical vein endothelial cells
Chromatin immunoprecipitation assay.
This work was supported by Chang Gung Memorial Hospital and Chang Gung Institute of Technology Chiayi Campus (grants CZRPG880253, CMRPG690171, CMRPG6A0061, CMRPG6A0011, CMRPF680052 and EZRPF390261).
- Gallagher DJ, Kemeny N: Metastatic colorectal cancer: from improved survival to potential cure. Oncology. 2010, 78: 237-248. 10.1159/000315730.View ArticlePubMed
- Strieter RM: Chemokines: not just leukocyte chemoattractants in the promotion of cancer. Nat Immunol. 2001, 2: 285-286.View ArticlePubMed
- Vandercappellen J, Van Damme J, Struyf S: The role of CXC chemokines and their receptors in cancer. Cancer Lett. 2008, 267: 226-244. 10.1016/j.canlet.2008.04.050.View ArticlePubMed
- Burger JA, Kipps TJ: CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment. Blood. 2006, 107: 1761-1767. 10.1182/blood-2005-08-3182.View ArticlePubMed
- Teicher BA, Fricker SP: CXCL12 (SDF-1)/CXCR4 pathway in cancer. Clin Cancer Res. 2010, 16: 2927-2931. 10.1158/1078-0432.CCR-09-2329.View ArticlePubMed
- Kryczek I, Lange A, Mottram P, Alvarez X, Cheng P, Hogan M, Moons L, Wei S, Zou L, Machelon V, Emilie D, Terrassa M, Lackner A, Curiel TJ, Carmeliet P, Zou W: CXCL12 and vascular endothelial growth factor synergistically induce neoangiogenesis in human ovarian cancers. Cancer Res. 2005, 65: 465-472.PubMed
- Darash-Yahana M, Pikarsky E, Abramovitch R, Zeira E, Pal B, Karplus R, Beider K, Avniel S, Kasem S, Galun E, Peled A: Role of high expression levels of CXCR4 in tumor growth, vascularization, and metastasis. FASEB J. 2004, 18: 1240-1242.PubMed
- Ottaiano A, Franco R, Aiello Talamanca A, Liguori G, Tatangelo F, Delrio P, Nasti G, Barletta E, Facchini G, Daniele B, Di Blasi A, Napolitano M, Ieranò C, Calemma R, Leonardi E, Albino V, De Angelis V, Falanga M, Boccia V, Capuozzo M, Parisi V, Botti G, Castello G, Vincenzo Iaffaioli R, Scala S: Overexpression of both CXC chemokine receptor 4 and vascular endothelial growth factor proteins predicts early distant relapse in stage II-III colorectal cancer patients. Clin Cancer Res. 2006, 12: 2795-2803. 10.1158/1078-0432.CCR-05-2142.View ArticlePubMed
- Kim J, Takeuchi H, Lam ST, Turner RR, Wang HJ, Kuo C, Foshag L, Bilchik AJ, Hoon DS: Chemokine receptor CXCR4 expression in colorectal cancer patients increases the risk for recurrence and for poor survival. J Clin Oncol. 2005, 23: 2744-2753.View ArticlePubMed
- Paschos KA, Canovas D, Bird NC: The role of cell adhesion molecules in the progression of colorectal cancer and the development of liver metastasis. Cell Signal. 2009, 21: 665-674. 10.1016/j.cellsig.2009.01.006.View ArticlePubMed
- Ksiazek K, Mikuła-Pietrasik J, Catar R, Dworacki G, Winckiewicz M, Frydrychowicz M, Dragun D, Staniszewski R, Jörres A, Witowski J: Oxidative stress-dependent increase in ICAM-1 expression promotes adhesion of colorectal and pancreatic cancers to the senescent peritoneal mesothelium. Int J Cancer. 2010, 127: 293-303.PubMed
- Arteta B, Lasuen N, Lopategi A, Sveinbjörnsson B, Smedsrød B, Vidal-Vanaclocha F: Colon carcinoma cell interaction with liver sinusoidal endothelium inhibits organ-specific antitumor immunity through interleukin-1-induced mannose receptor in mice. Hepatology. 2010, 51: 2172-2182. 10.1002/hep.23590.View ArticlePubMed
- Boyano MD, Garcia-Vázquez MD, López-Michelena T, Gardeazabal J, Bilbao J, Cañavate ML, Galdeano AG, Izu R, Díaz-Ramón L, Raton JA, Díaz-Pérez JL: Soluble interleukin-2 receptor, intercellular adhesion molecule-1 and interleukin-10 serum levels in patients with melanoma. Br J Cancer. 2000, 83: 847-852. 10.1054/bjoc.2000.1402.PubMed CentralView ArticlePubMed
- Alexiou D, Karayiannakis AJ, Syrigos KN, Zbar A, Kremmyda A, Bramis I, Tsigris C: Serum levels of E-selectin, ICAM-1 and VCAM-1 in colorectal cancer patients: correlations with clinicopathological features, patient survival and tumour surgery. Eur J Cancer. 2001, 37: 2392-2397. 10.1016/S0959-8049(01)00318-5.View ArticlePubMed
- Dymicka-Piekarska V, Kemona H: Does colorectal cancer clinical advancement affect adhesion molecules (sP-selectin, sE-selectin and ICAM-1) concentration?. Thromb Res. 2009, 124: 80-83. 10.1016/j.thromres.2008.11.021.View ArticlePubMed
- Hayes SH, Seigel GM: Immunoreactivity of ICAM-1 in human tumors, metastases and normal tissues. Int J Clin Exp Pathol. 2009, 2: 553-560.PubMed CentralPubMed
- Taglia L, Matusiak D, Matkowskyj KA, Benia RV: Gastrin-releasing peptide mediates its morphogenic properties in human colon cancer by upregulating intracellular adhesion protein-1 (ICAM-1) via focal adhesion kinase. Am J Physiol Gastrointest Liver Physiol. 2007, 292: G182-G190.View ArticlePubMed
- Gulubova MV: Expression of cell adhesion molecules, their ligands and tumour necrosis factorα in the liver of patients with metastatic gastrointestinal carcinomas. Histochem J. 2002, 34: 67-77. 10.1023/A:1021304227369.View ArticlePubMed
- Yadav VR, Prasad S, Sung B, Gelovani JG, Guha S, Krishnan S, Aggarwal BB: Boswellic acid inhibits growth and metastasis of human colorectal cancer in orthotopic mouse model by downregulating inflammatory, proliferative, invasive and angiogenic biomarkers. Int J Cancer. 2012, 130: 2176-2784. 10.1002/ijc.26251.PubMed CentralView ArticlePubMed
- Huang WS, Chin CC, Chen CN, Kuo YH, Chen TC, Yu HR, Tung SY, Shen CH, Hsieh YY, Guo SE, Shi CS, Liu TJ, Kuo HC: Stromal cell-derived factor-1/CXC receptor 4 and β1 integrin interaction regulates urokinase-type plasminogen activator expression in human colorectal cancer cells. J Cell Physiol. 2012, 227: 1114-1122. 10.1002/jcp.22831.View ArticlePubMed
- Hartmann TN, Burger JA, Glodek A, Fujii N, Burger M: CXCR4 chemokine receptor and integrin signaling co-operate in mediating adhesion and chemoresistance in small cell lung cancer (SCLC) cells. Oncogene. 2005, 24: 4462-4471. 10.1038/sj.onc.1208621.View ArticlePubMed
- Burger M, Glodek A, Hartmann T, Schmitt-Gräff A, Silberstein LE, Fujii N, Kipps TJ, Burger JA: Functional expression of CXCR4 (CD184) on small-cell lung cancer cells mediates migration, integrin activation, and adhesion to stromal cells. Oncogene. 2003, 22: 8093-8101. 10.1038/sj.onc.1207097.View ArticlePubMed
- Jones J, Marian D, Weich E, Engl T, Wedel S, Relja B, Jonas D, Blaheta RA: CXCR4 chemokine receptor engagement modifies integrin dependent adhesion of renal carcinoma cells. Exp Cell Res. 2007, 313: 4051-4065. 10.1016/j.yexcr.2007.07.001.View ArticlePubMed
- Chen YY, Malik M, Tomkowicz BE, Collman RG, Ptasznik A: BCR-ABL1 alters SDF-1α-mediated adhesive responses through the β2 integrin LFA-1 in leukemia cells. Blood. 2008, 111: 5182-5186. 10.1182/blood-2007-10-117705.PubMed CentralView ArticlePubMed
- Peled A, Grabovsky V, Habler L, Sandbank J, Arenzana-Seisdedos F, Petit I, Ben-Hur H, Lapidot T, Alon R: The chemokine SDF-1 stimulates integrin-mediated arrest of CD34+ cells on vascular endothelium under shear flow. J Clin Invest. 1999, 104: 1199-1211. 10.1172/JCI7615.PubMed CentralView ArticlePubMed
- Arlt A, Schäfer H: Role of the immediate early response 3 (IER3) gene in cellular stress response, inflammation and tumorigenesis. Eur J Cell Biol. 2011, 90: 545-552. 10.1016/j.ejcb.2010.10.002.View ArticlePubMed
- Zhu C, Qi X, Chen Y, Sun B, Dai Y, Gu Y: PI3K/Akt and MAPK/ERK1/2 signaling pathways are involved in IGF-1-induced VEGF-C upregulation in breast cancer. J Cancer Res Clin Oncol. 2011, 137: 1587-1594. 10.1007/s00432-011-1049-2.View ArticlePubMed
- Chen CN, Chang SF, Lee PL, Chang K, Chen LJ, Usami S, Chien S, Chiu JJ: Neutrophils, lymphocytes, and monocytes exhibit diverse behaviors in transendothelial and subendothelial migrations under coculture with smooth muscle cells in disturbed flow. Blood. 2006, 107: 1933-1942. 10.1182/blood-2005-08-3137.PubMed CentralView ArticlePubMed
- Chen TC, Chien SJ, Kuo HC, Huang WS, Sheen JM, Lin TH, Yen CK, Sung ML, Chen CN: High glucose-treated macrophages augment E-selectin expression in endothelial cells. J Biol Chem. 2011, 286: 25564-25573. 10.1074/jbc.M111.230540.PubMed CentralView ArticlePubMed
- Yeh CC, Chang HI, Chiang JK, Tsai WT, Chen LM, Wu CP, Chien S, Chen CN: Regulation of plasminogen activator inhibitor-1 expression in osteoarthritic chondrocytes by fluid shear stress: role of PKCα. Arthritis Rheum. 2009, 60: 2350-2361. 10.1002/art.24680.View ArticlePubMed
- Kobayashi H, Boelte KC, Lin PC: Endothelial cell adhesion molecules and cancer progression. Curr Med Chem. 2007, 14: 377-386. 10.2174/092986707779941032.View ArticlePubMed
- ten Kate M, Hofland LJ, van Grevenstein WM, van Koetsveld PV, Jeekel J, van Eijck CH: Influence of proinflammatory cytokines on the adhesion of human colon carcinoma cells to lung microvascular endothelium. Int J Cancer. 2004, 112: 943-950. 10.1002/ijc.20506.View ArticlePubMed
- Kang X, Wang F, Xie JD, Cao J, Xian PZ: Clinical evaluation of serum concentrations of intercellular adhesion molecule-1 in patients with colorectal cancer. World J Gastroenterol. 2005, 11: 4250-4253.PubMed CentralView ArticlePubMed
- Maeda K, Kang SM, Sawada T, Nishiguchi Y, Yashiro M, Ogawa Y, Ohira M, Ishikawa T, Hirakawa-YS Chung K: Expression of intercellular adhesion molecule-1 and prognosis in colorectal cancer. Oncol Rep. 2002, 9: 511-514.PubMed
- Koizumi K, Hojo S, Akashi T, Yasumoto K, Saiki I: Chemokine receptors in cancer metastasis and cancer cell-derived chemokines in host immune response. Cancer Sci. 2007, 98: 1652-1658. 10.1111/j.1349-7006.2007.00606.x.View ArticlePubMed
- Chu CY, Cha ST, Lin WC, Lu PH, Tan CT, Chang CC, Lin BR, Jee SH, Kuo ML: Stromal cell-derived factor-1α (SDF-1α/CXCL12)-enhanced angiogenesis of human basal cell carcinoma cells involves ERK1/2-NF-κB/interleukin-6 pathway. Carcinogenesis. 2009, 30: 205-213.View ArticlePubMed
- Ratajczak MZ, Zuba-Surma E, Kucia M, Reca R, Wojakowski W, Ratajczak J: The pleiotropic effects of the SDF-1-CXCR4 axis in organogenesis, regeneration and tumorigenesis. Leukemia. 2006, 20: 1915-1924. 10.1038/sj.leu.2404357.View ArticlePubMed
- Scotton CJ, Wilson JL, Scott K, Stamp G, Wilbanks GD, Fricker S, Bridger G, Balkwill FR: Multiple actions of the chemokine CXCL12 on epithelial tumor cells in human ovarian cancer. Cancer Res. 2002, 62: 5930-5938.PubMed
- Hassan S, Baccarelli A, Salvucci O, Basik M: Plasma stromal cell-derived factor-1: host derived marker predictive of distant metastasis in breast cancer. Clin Cancer Res. 2008, 14: 446-454. 10.1158/1078-0432.CCR-07-1189.View ArticlePubMed
- Akishima-Fukasawa Y, Nakanishi Y, Ino Y, Moriya Y, Kanai Y, Hirohashi S: Prognostic significance of CXCL12 expression in patients with colorectal carcinoma. Am J Clin Pathol. 2009, 132: 202-210. 10.1309/AJCPK35VZJEWCUTL.View ArticlePubMed
- Kollmar O, Rupertus K, Scheuer C, Junker B, Tilton B, Schilling MK, Menger MD: Stromal cell-derived factor-1 promotes cell migration and tumor growth of colorectal metastasis. Neoplasia. 2007, 9: 862-870. 10.1593/neo.07559.PubMed CentralView ArticlePubMed
- Roebuck KA, Finnegan A: Regulation of intercellular adhesion molecule-1 (CD54) gene expression. J Leukoc Biol. 1999, 66: 876-888.PubMed
- Chen JS, Hsu YM, Chen CC, Chen LL, Lee CC, Huang TS: Secreted heat shock protein 90α induces colorectal cancer cell invasion through CD91/LRP-1 and NF-κB-mediated integrin αV expression. J Biol Chem. 2010, 285: 25458-25466. 10.1074/jbc.M110.139345.PubMed CentralView ArticlePubMed
- Chun KS, Surh YJ: Signal transduction pathways regulating cyclooxygenase-2 expression: potential molecular targets for chemoprevention. Biochem Pharmacol. 2004, 68: 1089-1100. 10.1016/j.bcp.2004.05.031.View ArticlePubMed
- John AE, Zhu YM, Brightling CE, Pang L, Knox AJ: Human airway smooth muscle cells from asthmatic individuals have CXCL8 hypersecretion due to increased NF-κB p65, C/EBP β, and RNA polymerase II binding to the CXCL8 promoter. J Immunol. 2009, 183: 4682-4692. 10.4049/jimmunol.0803832.PubMed CentralView ArticlePubMed
- Richer E, Campion CG, Dabbas B, White JH, Cellier MF: Transcription factors Sp1 and C/EBP regulate NRAMP1 gene expression. FEBS J. 2008, 275: 5074-5089. 10.1111/j.1742-4658.2008.06640.x.View ArticlePubMed
- Kao YS, Fong JC: Endothelin-1 induces glut1 transcription through enhanced interaction between Sp1 and NF-κB transcription factors. Cell Signal. 2008, 20: 771-778. 10.1016/j.cellsig.2007.12.012.View ArticlePubMed
- Patel DN, King CA, Bailey SR, Holt JW, Venkatachalam K, Agrawal A, Valente AJ, Chandrasekar B: Interleukin-17 stimulates C-reactive protein expression in hepatocytes and smooth muscle cells via p38 MAPK and ERK1/2-dependent NF-κB and C/EBPβ activation. J Biol Chem. 2007, 282: 27229-27238. 10.1074/jbc.M703250200.View ArticlePubMed
- Xu H, Wu Q, Dang S, Jin M, Xu J, Cheng Y, Pan M, Wu Y, Zhang C, Zhang Y: Alteration of CXCR7 expression mediated by TLR4 promotes tumor cell proliferation and migration in human colorectal carcinoma. PLoS One. 2011, 6: e27399-10.1371/journal.pone.0027399.PubMed CentralView ArticlePubMed
- Kawada K, Hosogi H, Sonoshita M, Sakashita H, Manabe T, Shimahara Y, Sakai Y, Takabayashi A, Oshima M, Taketo MM: Chemokine receptor CXCR3 promotes colon cancer metastasis to lymph nodes. Oncogene. 2007, 26: 4679-4688. 10.1038/sj.onc.1210267.View ArticlePubMed
- Brühl H, Cohen CD, Linder S, Kretzler M, Schlöndorff D, Mack M: Post-translational and cell type-specific regulation of CXCR4 expression by cytokines. Eur J Immunol. 2003, 33: 3028-3037. 10.1002/eji.200324163.View ArticlePubMed
- Levoye A, Balabanian K, Baleux F, Bachelerie F, Lagane B: CXCR7 heterodimerizes with CXCR4 and regulates CXCL12-mediated G protein signaling. Blood. 2009, 113: 6085-6093. 10.1182/blood-2008-12-196618.View ArticlePubMed
- Burns JM, Summers BC, Wang Y, Melikian A, Berahovich R, Miao Z, Penfold ME, Sunshine MJ, Littman DR, Kuo CJ, Wei K, McMaster BE, Wright K, Howard MC, Schall TJ: A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development. J Exp Med. 2006, 203: 2201-2213. 10.1084/jem.20052144.PubMed CentralView ArticlePubMed
- Finzel AH, Reininger AJ, Bode PA, Wurzinger LJ: ICAM-1 supports adhesion of human small-cell lung carcinoma to endothelial cells. Clin Exp Metastasis. 2004, 21: 185-189.View ArticlePubMed
- Kawai Y, Kaidoh M, Yokoyama Y, Sano K, Ohhashi T: Chemokine CCL2 facilitates ICAM-1-mediated interactions of cancer cells and lymphatic endothelial cells in sentinel lymph nodes. Cancer Sci. 2009, 100: 419-428. 10.1111/j.1349-7006.2008.01064.x.View ArticlePubMed
- Roland CL, Harken AH, Sarr MG, Barnett CC: ICAM-1 expression determines malignant potential of cancer. Surgery. 2007, 141: 705-707. 10.1016/j.surg.2007.01.016.View ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.