Figure 6

Anandamide has no effects on inwardly rectifying K⁺ current I _K1 and delayed rectifier K⁺ current I _K . A: typical current recordings from the same cell in Tyrode (on top left panel), in the presence of 3 μM Anandamide (middle), and in the presence of both anandamide and 100 μM Ba²⁺ (bottom). The K⁺ currents were evoked by applying 400 ms depolarizing steps from -120 to +50 mV with an interval of 10 mV from a holding potential of -40 mV. B. I-V relationships before (open square), after addition of 3 μM anandamide (black square), and Ba²⁺ and anandamide together (black triangle). The asterisks indicate significance. The data were averaged from 5 cells. C: Summarized data (n = 11) of inwardly rectifying K⁺ currents at -80 mV and the effects of anandamide and Ba²⁺. The horizontal bar indicates the drug administration. The blank column is the control in Tyrode, anandamide alone (black column), and Ba²⁺ and anandamide together (column with back slash). Anandamide had no effect on K⁺ currents in ED11 ventricular myocytes. The inset of Fig. 6C summarizes the anandamide and Ba²⁺ sensitive currents. The data were presented as mean ± SE from the same cells (n = 11).