Down-regulation of intracellular adhesion molecules E-cadherin and -catenin in NPC-BM clones. E-cadherin and β-catenin in cell lysates of NPC-BM00, NPC-BM29 and NPC-BM18 were analyzed by Western blot using monoclonal antibodies specific for these molecules. Specific Western blot signals were visualized on an X-ray film by incubating with ECL-Plus chemiluminescence reagents. Panel A, E-cadherin; panel B, -catenin. The expression of E-cadherin remained unchanged in cells of different passages. Upregulation of transcriptional factor Ets1 was detected in NPC-BM29 cells (panel C). Both NPC-BM00 and NPC-BM29 cells were processed for Western blot analysis the same way as done for E-cadherin and -catenin except the monoclonal antibody specific for Ets1 was used.