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Table 1 Immunophenotypic characteristics of two NPC variants BM-00 and BM-29 isolated from the NPC-BM1 cell line.

From: Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome

Monoclonal antibody

BM-00

BM-29

Cell surface

Cytoplasmic

Cell surface

Cytoplasmic

HLA-A, B, C

96.6 (70.0)

99.5 (98.8)

98.2 (52.9)

93.2 (40.1)

HLA-DR

37.9 (43.7)

44.2 (38.9)

0.16 (42.3)

0.8

NMIgG

1.0

1.6

1.0

0.4

PBS

0.9

0.6

0.9

1.0

EMA

8.0

82.7 (24.5)

5.9

75.2 (18.9)

AE1

ND

54.5 (36.5)

ND

40.5 (23.2)

AE3

ND

28.5 (9.7)

ND

44.2 (8.4)

MAK6

ND

17.8 (9.7)

ND

28.7 (8.5)

CK7

ND

97.6 (57.8)

ND

99.9 (55.1)

CK20

ND

37.7 (13.7)

ND

31.8 (11.4)

EpCAM (ESA)

98.7 (54.5)

ND

93.8 (39.3)

ND

Sialyl-Tn

2.6

5.2

7.8

34.1 (14.5)

Ley

0.5

1.2

0.8

0.8

BH8.23(50-55 kDa)

6.5

77.3 (32.5)

6.4

95.4 (33.8)

CEA

ND

9.5

ND

4.8

Vimentin

ND

97.7 (84.6)

ND

99.9 (92.1)

VEGF

ND

98.7 (29.4)

ND

99.7 (31.8)

CD44s

89.7 (20.3)

ND

90.0 (26.8)

ND

CD54

95.7 (36.3)

ND

87.6 (23.8)

ND

CD58

80.8 (10.6)

ND

99.2 (31.0)

ND

  1. Results are obtained by immunofluorescence and flow cytometric analysis and are expressed as % positive cells and relative mean fluorescence intensity (in the bracket). Note that those results with <10% positive cells are considered negative and thus values of mean fluorescence intensity are not given.
  2. Abbreviations: ND, not determined; NMIgG, normal mouse Ig; PBS, phosphate-buffered saline; EMA, epithelial membrane antigen; CK, cytokeratin, EpCAM, epithelial cellular adhesion molecule; ESA, epithelial specific antigen; Ley. Lewis Y; CEA, embryonic carcinoma antigen; VEGF, vascular endothelial growth factor.