Skip to main content

Table 1 Immunophenotypic characteristics of two NPC variants BM-00 and BM-29 isolated from the NPC-BM1 cell line.

From: Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome

Monoclonal antibody BM-00 BM-29
Cell surface Cytoplasmic Cell surface Cytoplasmic
HLA-A, B, C 96.6 (70.0) 99.5 (98.8) 98.2 (52.9) 93.2 (40.1)
HLA-DR 37.9 (43.7) 44.2 (38.9) 0.16 (42.3) 0.8
NMIgG 1.0 1.6 1.0 0.4
PBS 0.9 0.6 0.9 1.0
EMA 8.0 82.7 (24.5) 5.9 75.2 (18.9)
AE1 ND 54.5 (36.5) ND 40.5 (23.2)
AE3 ND 28.5 (9.7) ND 44.2 (8.4)
MAK6 ND 17.8 (9.7) ND 28.7 (8.5)
CK7 ND 97.6 (57.8) ND 99.9 (55.1)
CK20 ND 37.7 (13.7) ND 31.8 (11.4)
EpCAM (ESA) 98.7 (54.5) ND 93.8 (39.3) ND
Sialyl-Tn 2.6 5.2 7.8 34.1 (14.5)
Ley 0.5 1.2 0.8 0.8
BH8.23(50-55 kDa) 6.5 77.3 (32.5) 6.4 95.4 (33.8)
CEA ND 9.5 ND 4.8
Vimentin ND 97.7 (84.6) ND 99.9 (92.1)
VEGF ND 98.7 (29.4) ND 99.7 (31.8)
CD44s 89.7 (20.3) ND 90.0 (26.8) ND
CD54 95.7 (36.3) ND 87.6 (23.8) ND
CD58 80.8 (10.6) ND 99.2 (31.0) ND
  1. Results are obtained by immunofluorescence and flow cytometric analysis and are expressed as % positive cells and relative mean fluorescence intensity (in the bracket). Note that those results with <10% positive cells are considered negative and thus values of mean fluorescence intensity are not given.
  2. Abbreviations: ND, not determined; NMIgG, normal mouse Ig; PBS, phosphate-buffered saline; EMA, epithelial membrane antigen; CK, cytokeratin, EpCAM, epithelial cellular adhesion molecule; ESA, epithelial specific antigen; Ley. Lewis Y; CEA, embryonic carcinoma antigen; VEGF, vascular endothelial growth factor.