Figure 1

Inhibition of bacterial internalization by the R2-25 antibody treatment. (A) The purified R2-25 antibody was added to the cell culture medium for the bacterial internalization assay at the indicated concentrations 2 h before infection. Data are the averages of triplicate samples from three identical experiments, and the error bars represent standard deviations. Statistically significant differences between bacterial internalization into TG cells treated with the R2-25 antibody and those treated with rat IgG are indicated by asterisks (*, P < 0.01). Immunofluorescence staining of B. abortus or L. monocytogenes (green) and Hsc70 (B) or Hsp90 (red) (C) in TG cells. Colocalized bacteria with Hsc70 are shown in yellow in the merged images. Arrows point to colocalized bacteria. Scale bar indicates 10 μm.