Figure 4

Shear-induced Nrf2 nuclear translocation is not affected by nitric oxide synthase (NOS) inhibitors. (A and B) Shear-induced NO is not involved in the Nrf2 nuclear translocation. HUVECs were (A) pretreated with L-NAME, an NOS inhibitor (300 μM), for 1 h or (B) pretreated with L-NNA, an NOS inhibitor (250 μM), for 1 h, and then kept as a static control or exposed to shear stress in the presence of the inhibitor for 2 h. Nuclear extracts were prepared and subjected to Western blotting with anti-Nrf2 and anti-nucleolin (internal control) antibodies. Similar results were obtained from repeated experiments.