Table 1 Secreted phospholipase A2 (sPLA2) activity of recombinant wild-type or mutated VP1-unique region (VP1u) proteins
From: Effects of human parvovirus B19 VP1 unique region protein on macrophage responses
Proteins | sPLA2 activity (μmol/min/mL) |
|---|---|
bvPLA2 (1 ng) | 0.56 ± 0.12 |
VP1u (0.5 ug) | 0.03 ± 0.02 |
VP1u (5 ug) | 0.19 ± 0.03 *,& |
VP1uD175A(0.5 ug) | ND |
VP1uD175A (5 ug) | ND |
Rabbit anti-VP1u IgG (0.5 ug) | ND |
Rabbit anti-VP1u IgG (5 ug) | ND |
Rabbit anti-VP1uD175A IgG (0.5 ug) | ND |
Rabbit anti-VP1uD175A IgG (5 ug) | ND |
Rabbit IgG (0.5 ug) | ND |
Rabbit IgG (5 ug) | ND |
Rabbit anti-NS1 IgG (0.5 ug) | ND |
Rabbit anti-NS1 IgG (5 ug) | ND |
VP1u (5 ug) + Rabbit IgG (0.5 ug) | 0.18 ± 0.07 |
VP1u (5 ug) + Rabbit IgG (5 ug) | 0.19 ± 0.06 |
VP1u (5 ug) + Rabbit anti-B19-VP1u IgG (0.5 ug) | 0.20 ± 0.11 |
VP1u (5 ug) + Rabbit anti-B19-VP1u IgG (5 ug) | 0.09 ± 0.03 # |
VP1u (5 ug) + Rabbit anti-B19-VP1uD175A IgG (0.5 ug) | 0.18 ± 0.05 |
VP1u (5 ug) + Rabbit anti-B19-VP1uD175A IgG (5 ug) | 0.16 ± 0.06 |
VP1u (5 ug) + Rabbit anti-B19-NS1 IgG (5 ug) | 0.16 ± 0.04 |
VP1uD175A (5 ug) + Rabbit anti-B19-VP1u IgG (5 ug) | ND |
VP1uD175A (5 ug) + Rabbit anti-B19-VP1uD175A IgG (5 ug) | ND |