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Table 1 Secreted phospholipase A2 (sPLA2) activity of recombinant wild-type or mutated VP1-unique region (VP1u) proteins

From: Effects of human parvovirus B19 VP1 unique region protein on macrophage responses

Proteins sPLA2 activity
(μmol/min/mL)
bvPLA2 (1 ng) 0.56 ± 0.12
VP1u (0.5 ug) 0.03 ± 0.02
VP1u (5 ug) 0.19 ± 0.03 *,&
VP1uD175A(0.5 ug) ND
VP1uD175A (5 ug) ND
Rabbit anti-VP1u IgG (0.5 ug) ND
Rabbit anti-VP1u IgG (5 ug) ND
Rabbit anti-VP1uD175A IgG (0.5 ug) ND
Rabbit anti-VP1uD175A IgG (5 ug) ND
Rabbit IgG (0.5 ug) ND
Rabbit IgG (5 ug) ND
Rabbit anti-NS1 IgG (0.5 ug) ND
Rabbit anti-NS1 IgG (5 ug) ND
VP1u (5 ug) + Rabbit IgG (0.5 ug) 0.18 ± 0.07
VP1u (5 ug) + Rabbit IgG (5 ug) 0.19 ± 0.06
VP1u (5 ug) + Rabbit anti-B19-VP1u IgG (0.5 ug) 0.20 ± 0.11
VP1u (5 ug) + Rabbit anti-B19-VP1u IgG (5 ug) 0.09 ± 0.03 #
VP1u (5 ug) + Rabbit anti-B19-VP1uD175A IgG (0.5 ug) 0.18 ± 0.05
VP1u (5 ug) + Rabbit anti-B19-VP1uD175A IgG (5 ug) 0.16 ± 0.06
VP1u (5 ug) + Rabbit anti-B19-NS1 IgG (5 ug) 0.16 ± 0.04
VP1uD175A (5 ug) + Rabbit anti-B19-VP1u IgG (5 ug) ND
VP1uD175A (5 ug) + Rabbit anti-B19-VP1uD175A IgG (5 ug) ND
  1. bvPLA2: sPLA2 from bee venom; D175A proteins encoded by genes with point mutations in the enzyme catalytic site
  2. ND means non-detected
  3. * and &indicate P < 0.05, compared to VP1u (0.5 ug) or VP1uD175A (5 ug), respectively.
  4. # indicates P < 0.05, compared to VP1u (5 ug).