Immunofluorescence of dengue infection and dominant negative mutant of Eps15. HepG2 cell were transfected with either of two dominant negative Eps15 mutants (DIII or EH29) or a wild type Eps15 clone (D3Δ2) all fused to GFP or pEGFP as control, followed by infection with each dengue virus serotype individually or Alexa 594 conjugated-transferrin. Dengue infected samples were subsequently incubated with a mouse monoclonal antibody directed against dengue E protein and an Alexa 594 conjugated chicken anti-mouse IgG antibody. Signal from Alexa 594 (red) and GFP (green) were observed under a fluorescent microscope. Merged images are shown.