Figure 4
From: Characterization of dengue virus entry into HepG2 cells
Immunofluorescence of dengue infection and dominant negative mutant of Eps15. HepG2 cell were transfected with either of two dominant negative Eps15 mutants (DIII or EH29) or a wild type Eps15 clone (D3Δ2) all fused to GFP or pEGFP as control, followed by infection with each dengue virus serotype individually or Alexa 594 conjugated-transferrin. Dengue infected samples were subsequently incubated with a mouse monoclonal antibody directed against dengue E protein and an Alexa 594 conjugated chicken anti-mouse IgG antibody. Signal from Alexa 594 (red) and GFP (green) were observed under a fluorescent microscope. Merged images are shown.