Figure 4

Effect of ischemic conditioning on mitochondrial cytochrome C (Mcytc) and cytosolic cytochrome C (Ccytc) (A), autophagy- (B), apoptosis- (C), and antioxidant-related (D) proteins in the rat kidney subjected to ischemia/reperfusion injury. I30 × 2 decreased Ccytc release when compared to I15 × 4 and I60 kidneys. In the autophagy-related protein expression, the expression in LC3 and Beclin-1 protein is implicated in an order of I30 × 2 > I15 × 4 > I60 > CN. In the proapoptotic mechanisms, increased Bax/Bcl-2 ratio, active CPP32 and PARP degradation expression are displayed in an order of I30 × 2 > I15 × 4 > I60 > CN. In the antioxidant protein expression, the expression in MnSOD, CuZnSOD amd catalase is demonstrated in an order of I30 × 2 > I15 × 4 > I60 > control (CN). *, P < 0.05 vs. CN group. #, P < 0.05 vs. I60 group.