Reduced percentage of DCs and impaired Flt3L-dependent DC development in P117. Splenocytes of STAT2+/m or STAT2m/m (P117) mice were stained with antibodies to CD11c, CD86, and B220 or CD11b. The percentage of pDC (CD11c+B220+) (A) or cDC (CD11c+CD11b+) (B) in STAT2+/m (upper panels) or STAT2m/m (P117) (lower panels) and the percentage of CD86 high gated on these two populations were shown (right panels). (C) The average percentage of cDC and pDC of STAT2+/m (empty bars) and STAT2m/m (P117) (solid bars) mice was shown (n = 3). * P < 0.05 BM-derived pDC and cDC from STAT2+/m (empty bars) and STAT2m/m (P117) (solid bars) mice were enumerated and analyzed by flow cytometry and the cellularity (D) and the percentage (E) were determined. pDC (F) and cDC (G) of STAT2+/m (upper panels) and STAT2m/m (P117) (lower panels) were then stimulated without (left panels) or with LPS plus R848 (right panels) for overnight and stained with anti-MHC class II, followed by flow cytometry.