Figure 1

Effects of genistein, EGF, 17β-estradiol and combinations of genistein with EGF or 17β-estradiol on GAG synthesis in MPS IIIA fibroblasts. Cells were untreated (Ctrl) or treated with genistein at concentrations 10 or 30 μM (G10 and G30, respectively) alone, EGF alone at 100 ng/ml (EGF), 17β-estradiol alone at 1 nM (Est) or in following combinations: genistein (10 or 30 μM) with EGF (100 ng/ml) (EGF+G10 and EGF+G30, respectively) and genistein (10 or 30 μM) with 17β-estradiol (1 nM) (Est+G10 and Est+G30, respectively). Cell cultures were labeled with 20 μCi/ml of [³⁵S]Na₂SO₄ for 24 h, and GAG synthesis was monitored by measuring incorporation of ³⁵S into proteoglycans. The results were calculated per DNA amount. The results are mean values of three experiments with error bars indicating SD.