Figure 2

Effects of genistein, EGF, 17β-estradiol and combinations of genistein with EGF or 17β-estradiol on accumulation of lysosomes containing complex storage structures in MPS IIIAfibroblasts. Control fibroblasts or MPS IIIA fibroblasts were untreated or treated for 4 days with genistein at concentrations 10 and 30 μM either alone or in combination with EGF (100 ng/ml) or 17β-estradiol (1 nM). Electron micrographs were performed at magnification 1650 x. Panels: (A), control (untreated wild-type cells); (B), untreated MPS IIIA cells; (C), MPS IIIA cells treated with 10 μM genistein; (D), MPS IIIA cells treated with 30 μM genistein; (E), MPS IIIA cells treated with 100 ng/ml EGF; (F), MPS IIIA cells treated with 100 ng/ml EGF and 10 μM genistein; (G), MPS IIIA cells treated with 100 ng/ml EGF and 30 μM genistein; (H), MPS IIIA cells treated with 1 nM 17β-estradiol; (I), MPS IIIA cells treated with 1 nM 17β-estradiol and 10 μM genistein; (J), MPS IIIA cells treated with 1 nM 17β-estradiol and 30 μM genistein.