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Figure 4 | Journal of Biomedical Science

Figure 4

From: Genistein-mediated inhibition of glycosaminoglycan synthesis, which corrects storage in cells of patients suffering from mucopolysaccharidoses, acts by influencing an epidermal growth factor-dependent pathway

Figure 4

Long-term effects of EGF and genistein on phosphorylation of the EGF receptor. Fibroblasts were cultured in the absence of EGF, which indicated the residual phosphorylation level (baseline value), and in the presence of EGF (100 ng/ml for 24 h) (Ctrl) without genistein or with genistein (either 10 or 30 μM, marked as G10 or G30, respectively) added together with EGF. Results of experiments with HDFa fibroblasts (wild-type) are shown. Similar effects were observed in experiments with MPS II, MPS IIIA and MPS IIIB fibroblasts (results not shown). The results were calculated as phospho-EGF receptor fluorescence at 600 nm normalized to the total EGF receptor fluorescence at 450 nm. Therefore, the obtained normalized values are in arbitrary units. Values represent mean ± range of duplicate determinations.

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