Skip to main content

Table 1 List of primers used for sequencing the entire CYP21A2

From: A novel 9-bp insertion detected in steroid 21-hydroxylase gene (CYP21A2): prediction of its structural and functional implications by computational methods

Primer Primer sequence Nucleotide Number Size Location
1F 5' aca gtc tac aca gca gga gg3' -78–(-59) 20 Promoter
1R 5' gtg agg gcc aga gcg aga t 3' 214–232 19 Intron 1
2F 5' gag gac cat tga tga agc 3' 322–339 18 Exon 2
2R 5' ctc aca gaa ctc ctg ggt ca 3' 806–825 20 Exon 3
4F 5' cct gtc ctt ggg aga cta ct 3' 700–719 20 Exon 3
4R 5' gtc cac aat ttg gat gga cca 3' 1187–1207 21 Exon 5
6R 5' agc aat gct gag gcc ggt ag 3' 1460–1479 20 Intron 6
7F 5' att gct atg agg cgg gtt c3' 1474–1492 19 Intron 6
8F 5' ctc act ggg ttg ctg agg gag 3' 1901–1921 21 Intron 7
9F 5' ctt cag cat ctc cgg cta c 3' 2238–2256 19 Intron 8
10R 5' ctg tgt tta cag ggg gga 3' 2778-2796 18 Intron 10