Figure 5From: Effects of statins on the secretion of human serum albumin in cultured HepG2 cellsEffects of simvastatin treatment on HSA secretion into culture media from HepG2 cells. HepG2 cells were treated with 0, 1, and 5 μM simvastatin for 22 hours in RPMI media. Cells were incubated additional 2 hours with [35S]-methionine/cysteine. Media was collected and the secreted HSA in the media was immunoprecipitated with a specific anti-HSA antibody followed by SDS-PAGE. Radioactive quantification of HSA bands was performed by liquid scintillation counting. Data are mean ± SD from three independent experiments performed in triplicate. *P < 0.05 vs. without simvastatin.Back to article page