Effects of simvastatin on total radiolabeled protein in HepG2 cells. Confluent HepG2 cells were treated with 1 and 5 μM simvastatin for 22 hours and then labeled for 2 hours with [35S]-methionine/cysteine. Total protein was determined by TCA precipitation. Data are mean ± SD from three independent experiments performed in triplicate. *P < 0.05 vs. without simvastatin.