Cells were incubated for 22 hours with 0, 1, and 5 μM simvastatin, then cell were pulse-labeled for 20 minutes with [35S] methionine. After pulse-labeling, media were washed and incubated for 20, 120 minutes in media containing an excess of unlabeled methionine/cysteine. At the end of each chase period, solubilized cell lysates and media were subjected to immunoprecipitation. Quantification of labeled HSA was determined by SDS-PAGE and scintillation counting. Data presented are from three separate experiments done in duplicate.
* Intra peaks were calculated from the HSA content at 20 minutes of chase
** % recovery represents total labeled apoB (cell lysate + media) remained after 2-hour chasing.