Skip to main content

Advertisement

Table 1 Effects of simvastatin on intracellular HSA degradation in HepG2 cells.

From: Effects of statins on the secretion of human serum albumin in cultured HepG2 cells

  HSA (102 × dpm)
  Control Simvastatin (1 μM) Simvastatin (5 μM)
Intra peak * 350 ± 31 414 ± 24 403 ± 52
Depletion 216 237 243
Secretion 168 173 143
Degradation 48 64 100
% Recovery ** 86.3 84.5 75.2
  1. Cells were incubated for 22 hours with 0, 1, and 5 μM simvastatin, then cell were pulse-labeled for 20 minutes with [35S] methionine. After pulse-labeling, media were washed and incubated for 20, 120 minutes in media containing an excess of unlabeled methionine/cysteine. At the end of each chase period, solubilized cell lysates and media were subjected to immunoprecipitation. Quantification of labeled HSA was determined by SDS-PAGE and scintillation counting. Data presented are from three separate experiments done in duplicate.
  2. * Intra peaks were calculated from the HSA content at 20 minutes of chase
  3. ** % recovery represents total labeled apoB (cell lysate + media) remained after 2-hour chasing.