Effects of D-GalN treatment on in vivo and in vitro ROS generation in the livers of rats. Increase in hepatic ROS occurred in D-Galn treated rats, but not in normal rats. Mean values of hepatic ROS and bile lucigenin and luminol ROS generation measured at different times after D-GalN treatment in rats are depicted. * P < 0.05 vs. control value (C). # P < 0.05 between D-GalN and normal groups.