A: The analysis of nuclear factor NF-κB and AP-1 from the nuclear extracts of liver samples at 6 hr of D-GalN treatment were performed with a nuclear extraction kit and using a commercially Electrophoretic-Mobility Shift Assay. The induction of nuclear factor NF-κB and AP-1 nuclear factors by D-GalN treatment is partly inhibited by green tea (GT) pretreatment.B: Immunoblot analyses for specific antibodies to ICAM-1, and β-actin were performed in livers taken at 6 hr after D-GalN treatment. Note the increased expression of ICAM-1 in response to D-GalN. C: The enhanced expressions of these specific proteins were significantly reduced by GT pretreatment. Equal protein loading was displayed by β-actin. * P < 0.05 vs. control value. # P < 0.05 vs. D-GalN treatment.