Characterization of luciferase expression in mice injected intradermally with luciferase expressing DNA with or without laser treatment. C57BL/6 mice (3 per group) were injected intradermally with 10 μg/mouse pcDNA3-luc in 50 μl volume. Immediately after DNA injection, the injected mice were treated with laser for 80 sec at a laser power density of 0.04 GW/cm2. Mice were imaged 16 hours after DNA injection using the IVIS Imaging System Series 200. Bioluminescence signals were acquired for 1 minute. (A) Representative luminescence images of injected mice with or without laser treatment. (B) Bar graph showing relative luciferase activity for both groups of mice using data from all mice tested (p < 0.05).