Figure 2
Representative graph showing displacement analysis of [3H]QNB binding against atropine in the hypothalamus of Control, Diabetic and Diabetic+Insulin treated group rats. Control (black circle), Diabetic (upward pointing black triangle), Insulin treated diabetic rats (downward pointing black triangle). Competition studies were carried out with 1 nM [3H]QNB in each tube with atropine concentrations varying from 10-9, 10-4 M. Data were fitted with iterative nonlinear regression software (Prism, GraphPad, San Diego, CA). Ki The affinity of the receptor for the competing drug. EC50 is the concentration of the competitor that competes for half the specific binding.