GAPDH and NS5 proteins colocalize within the JEV-infected cells. Double staining of GAPDH (red) and NS5 (green) proteins in BHK-21 (A) or HEK293 cells (B) was done as described in Materials and Methods. Cells were stained as uninfected (Uninf.) or at hours postinfection (hpi). Colocalization of both NS5 and GAPDH was evidenced by merged images (yellow). The scale bar is 40 μM.