No direct interaction of NS5 and GAPDH proteins was demonstrated by co-immunoprecipitation (Co-IP) followed by Western blot analyses against NS5 (A) or GAPDH (B). Lanes 1 and 2, 10 μg of uninfected (un.) and infected (inf.) cell extracts without (W/O) IP. Antibodies against various proteins for IP or no antibody added are indicated on the top.