Figure 4
In vitro interaction of GAPDH with plus-strand (A) and minus-strand (B) of JEV riboprobes. Lane 1, free probe (F). A. GAPDH (50 ng, 200 ng, 1 μg; lanes 2–4), or BSA (1 μg; lane 5) were added to the binding reaction as indicated on the top. B. GAPDH proteins (55 ng, 165 ng, 550 ng) without (lanes 2–4) or with 70 μM of H2O2 treated (lanes 5–7) were added to the binding reaction. RNA-protein complexes (RPC) are indicated by arrows.