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Figure 1 | Journal of Biomedical Science

Figure 1

From: Use of serial analysis of gene expression to reveal the specific regulation of gene expression profile in asthmatic rats treated by acupuncture

Figure 1

Protocol and measurements for the rat model of allergic asthma. Male Sprague-Dawley rats were sensitized intraperitoneally at day 0 with 1 mg of ovalbumin (OVA) precipitated with 10 mg of aluminium hydroxide gel in 1 ml normal saline. Manual acupuncture was performed regularly from the first day after the sensitization and given every other day over a period of two weeks. On day 14, the sensitized animals were challenged with 1 ml/kg of 5% OVA in normal saline (5 mg/kg) by injection into the external jugular vein over 10 s. The respiratory parameters of pulmonary resistance, dynamic compliance and respiratory rate were immediately recorded for 10 min. Rats were killed immediately after the measurement. Rats of CK and CKAC were sensitized and challenged with normal saline instead of OVA. Rats of CKAC received the same acupuncture treatment as those of ASAC.

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