Figure 4

Binding of ANS to apoNCS at various concentrations of GdnHCl. Fluorescent emission intensity of ANS (100 μM) in the presence of apoNCS (10 μM) was followed at 25°C in different concentration of GdnHCl using an excitation wavelength at 355 nm. Samples were prepared in 10 mM phosphate buffer at pH 3. Inset shows emission spectra of ANS with apoNCS in native (N, in 0 M GdnHCl), intermediate (I, in 1.2 M GdnHCl) and denatured (D, in 4 M GdnHCl) state.