Characteristics of cyst and mass clones cultured on dish. (a) Morphology of cyst clones (CA4, CA6) and mass clones (M610, M611, M612) cultured on dish for 24 hrs. Images were taken under a light microscope. Bar = 30 μm. (b) Growth curve of cyst and mass clones cultured on dish for 10 days. (c) Cyst clones and mass clones subjected to migration assays using collagen as a chemoattractant. Migrated cells were fixed, stained, and counted under a light microscope. Values (mean ± S.E.) are from 15 fields and 3 independent experiments.