Figure 4
From: Requirement of focal adhesion kinase in branching tubulogenesis
Comparison of FAK and FAK phosphorylation levels between cyst and mass clones cultured in 0.1% collagen gel. (a) Cyst clones (CA4, CA6) and mass clones (M610, M611, M612) were cultured in 0.1% collagen gel for indicated time. Lysates were analyzed by Western blot using antibodies to detect FAK and β-actin. (b) Cyst clones (CA4, CA6) and mass clones (M610, M611, M612) were cultured in 0.1% collagen gel for 6 days. Lysates were analyzed by Western blot using antibodies to detect FAK and Y397, Y406, Y577, Y861, Y925 phosphorylated FAK.