Figure 5

Morphology and migration ability of FAK transfectants in cyst and mass clones. (a-b) CA6, M610, control clones (CV, MV), wild type FAK overexpressed-CA6 cell (CF5), or antisense FAK overexpressed-M610 cells (MF^-9, MF^-13) were cultured on dish for 24 hrs. (a) Lysates were analyzed by Western blot using antibodies to detect FAK and β-actin. (b) Cell morphology; images were taken under a light microscope. Bar = 30 μm. (c) Cells were subjected to migration assays using collagen as a chemoattractant. Migrated cells were fixed, stained, and counted under a light microscope. Values (mean ± S.E.) are from 15 fields and 3 independent experiments.