Figure 6

Characteristics of FAK transfectants in cyst and mass clones cultured in 0.1% collagen gel. (a-b) CA6, M610, control clones (CV, MV), wild type FAK overexpressed-CA6 cell (CF5), or antisense FAK overexpressed-M610 cells (MF^-9, MF^-13) were cultured in 0.1% collagen gel for 6 days. (a) Morphology of cells, images were taken under a light microscope. Bar = 100 μm. (b) Lysates were analyzed by Western blot using antibodies to detect FAK, Y397 phosphorylated FAK and β-actin. (c) CA6, M610, control clones (CV, MV), wild type FAK overexpressed-CA6 cell (CF5), or antisense FAK overexpressed-M610 cells (MF^-9, MF^-13) were cultured on dish for 24 hrs or in 0.1% collagen gel for 6 days. Assembled fibronectin was extracted and analyzed by Western blot using antibodies to detect fibronectin.