Figure 8

TNF-α stimulates secretion of resistin from macrophages and reduces glucose uptake. (A) TNF-α at 1 ng/mL significantly increasesd release of resistin from cultured macropghes at different stimulation periods. SP600125 and atorvastatin attenuated the effect induced by TNF-α (n = 3). *P < 0.01 vs. control. **P < 0.001 vs. control.⁺P < 0.001 vs. 18 hr. (B) Resistin and conditioned medium (CM) after TNF-α treatment had similar effect on reducing glucose uptake. Glucose uptake was measured in macrophages treated for 90 min with 20 μg/mL recombinant resisitn protein or conditioned medium from cultured macrophages after 1 ng/mL TNF-α stimulation with or without TNF-α antibody (TNF-α Ab). *P < 0.05 vs. control. **P < 0.01 vs. control. Data are from 3 independent experiments.