Figure 2

Asynchronous mitosis occurs in mars and tlk / mars embryos at the non-permissive temperature. Embryos from tlk^Δ14/+ (A and B), mars^P(C and D) and tlk^Δ14/+; mars^P(E) females crossed with mars^Pmales were immunostained as described in the legend of Figure 4. In panel B, chromosomes aligned at spindle midzone before entering anaphase are indicated by a bracket above the embryo. Nuclei with delayed mitotic progression relative to neighboring nuclei at metaphase (C) or anaphase (D) are indicated by asterisks. The chromosome segregation in the tlk/mars embryos is very asynchronous (E). A white circle indicates a nucleus that is likely at metaphase based on the morphology of chromosomes. The scale bars without specification are 10 μm