Figure 4
From: mars and tousled-like kinase act in parallel to ensure chromosome fidelity in Drosophila
Embryos with reduced mars activity exhibit shorter and less dense mitotic spindles. (A) Immunoblotting of Mars protein extracted from 30 syncytial blastoderm embryos at metaphase with an anti-Mars antibody. A 135-kDa protein was detected and is labeled as Mars on the right. α-Tub was served as the loading control. (B-G) Embryos at 28.5°C were immunostained with anti-α-Tub (green) and anti-γ-Tub (green) and anti-phospho-histone H3 (red) antibodies. The morphology of the mitotic spindles at metaphase, anaphase and telophase in w1118(B-D) and mars (E-G) embryos are shown. The scale bar is 5 μm