Figure 5
From: mars and tousled-like kinase act in parallel to ensure chromosome fidelity in Drosophila
Mitotic defects in embryos with tlk overexpression are similar to those observed in mars embryos. Embryos with either GFP (GCN4>GFP) or tlk overexpression (GCN4>tlk) at 28.5°C were immunostained with anti-α-Tub (green) and anti-γ-Tub (green) antibodies. The chromosomes were stained by Hoechst 33342 (red). (A) GFP overexpression in metaphase embryo serves as a negative control. (B) Most of the mitotic spindles were normal in metaphase embryos when tlk was overexpressed. A few nuclei showed corrupted mitotic spindles that also appear to have less chromosomal DNA and these are indicated by asterisks. (C) A patch of nuclei with less condensed chromosomes at anaphase is indicated by arrows and the chromosomes seem to remain unseparated. These two phenotypes were also observed in GCN4>tlk embryos at 24°C. The scale bars are 10 μm.