Figure 7
From: mars and tousled-like kinase act in parallel to ensure chromosome fidelity in Drosophila
The M-phase arrest induced by mars overexpression is suppressed by tlk overexpression. Eye-antennal discs were dissected from the third-instar larvae and immunostained with anti-phospho-histone H3 antibody (green) and phalloidin (red) to label the M-phase cells and the neuronal clusters, respectively. The genotypes for panel A to D are w1118(A), GMR>mars (B), GMR>tlk (C)and GMR>mars tlk (D). The anterior of the discs is arranged toward the left. The arrows indicate the morphogenetic furrows (MF). The region behind the MF is divided into three distinct domains, namely I (4–6), II (7–10) and III (>11 rows of neuronal clusters), as described by Baker and Yu (2001) [45]. The color codes for the three domains are blue, green and red for domains I, II and III, respectively. (E) From each disc, the M-phase cells in two hundred clusters (10 clusters parallel and 20 clusters perpendicular to MF) were measured and plotted as a histogram. The representation and number of discs measured for each column are shown on the right. The statistical significance of M-phase cells presenting in the three regions was determined using the Student's t-test. The numbers of M-phase cells in the eye discs with overexpression of both mars and tlk differ significantly from those overexpressing mars or tlk alone and are also different from the w1118discs. These differences are indicated by asterisks and #, respectively (p < 0.05).