N-ethyl-N-nitrosourea mutagenesis produced a small number of mice with altered plasma electrolyte levels

Table 2 Retrospective analysis of the physiologic range of plasma electrolytes (mmol/l) in three-month-old C3H mice of the Munich ENU project

		Roche measurement equipment^a				Olympus measurement equipment^a
Electrolyte	Sex	n^b	Median	95% Range^c	90% Range	n^b	Median	95% Range^c	90% Range
Ca	m	6142	2.2	1.9–2.5	2.0–2.5	3945	2.2	1.8–2.4	2.0–2.4
Ca	f	4411	2.3	2.0–2.6	2.1–2.5	2152	2.2	2.0–2.5	2.0–2.4
Cl	m	6145	108	88–122	92–118	3345	115	104–126	106–124
Cl	f	4418	108	88–124	92–122	1836	115	102–125	106–123
K	m	6145	4.7	3.8–5.8	3.9–5.6	3345	5.0	4.0–6.0	4.2–5.8
K	f	4420	4.3	3.5–5.5	3.7–5.2	1836	4.6	3.8–5.8	4.0–5.6
Na	m	6146	152	138–164	140–162	3345	158	144–172	146–168
Na	f	4419	152	138–166	142–162	1836	156	144–172	146–168
P	m	6145	2.1	1.5–2.9	1.6–2.8	3923	1.8	1.4–2.6	1.4–2.4
P	f	4416	2.3	1.6–3.0	1.7–2.9	2151	2.0	1.4–2.6	1.4–2.6

m, male; f, female.
^a Plasma electrolyte levels were analyzed using the Roche Hitachi 717 autoanalyzer and the reagents for human samples, and subsequently the Olympus AU400 autoanalyzer and the reagents for human samples.
^b G1 and G3 offspring of ENU-treated mice for the dominant and recessive screen. About 60% of the analyzed mice were males.
^c Phenotypic variants were defined by two measurements of a three-week time period showing values below/above the lower/upper limit of the 95% range of the respective parameter.

ISSN: 1423-0127