Figure 3From: ZAK negatively regulates RhoGDIβ-induced Rac1-mediated hypertrophic growth and cell migrationZAK reverses the effects of RhoGDIβ on the induction of cell migration in H9c2 cells. (A) Wound healing assay. H9c2 cells and H9c2 cells ectopically expressing ZAK, dominant-negative ZAK (ZAKdn), RhoGDIβ, ZAK and RhoGDIβ, or ZAKdn and RhoGDIβ were seeded onto plates. After reaching confluency, the cell layer was wounded with a 200 μl pipette tip and incubated for 72 h with medium and doxycycline. (B) The cell migration capacity at 36 h was estimated by measuring the percentage wound closure (WC%). Values are means (SEM from three independent experiments).Back to article page