ZAK reverses the effects of RhoGDIβ on the induction of cell migration in H9c2 cells. (A) Wound healing assay. H9c2 cells and H9c2 cells ectopically expressing ZAK, dominant-negative ZAK (ZAKdn), RhoGDIβ, ZAK and RhoGDIβ, or ZAKdn and RhoGDIβ were seeded onto plates. After reaching confluency, the cell layer was wounded with a 200 μl pipette tip and incubated for 72 h with medium and doxycycline. (B) The cell migration capacity at 36 h was estimated by measuring the percentage wound closure (WC%). Values are means (SEM from three independent experiments).