Effects of PKC inhibitors with or without LPC on ACh-induced increases in [Ca2+]
. Fura-2 loaded endothelial cells were treated with the protein kinase C inhibitors Ro-31-8220 and calphostin C for 30 min. ACh (3 μmol/L) was added before and after treatment. Data are expressed as percentage value of ACh-induced increases in calcium prior to treatment. Each value represents the mean ± S.E. of 7 cells. **P < 0.01, ***P < 0.001 compared between indicated groups.