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Figure 1 | Journal of Biomedical Science

Figure 1

From: Mutagenesis identifies the critical amino acid residues of human endonuclease G involved in catalysis, magnesium coordination, and substrate specificity

Figure 1

Chemical modification of human EndoG. EndoG (0.2 pmol) was mixed with various amounts of DPEC and incubated at 25°C for 30 min. The residual activity was analyzed by standard assay conditions (lanes 1 to 4). An assay for restoration with hydroxylamine was performed by incubating 20 mM hydroxylamine and DEPC-treated EndoG together at 4°C for 5 h. The residual activity was then analyzed (lanes 6 to 9). Lane 5 represents the reaction performed in the absence of EndoG. Arrowheads denote the different topological forms of pUC18 plasmids. OC: open circular; L: linear; SC: supercoiled.

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Journal of Biomedical Science

ISSN: 1423-0127